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Highlights
- Depletes Host and Enriches for Bacteria: Increase the ratio of bacteria to host DNA.
- Fast: Only 30 minutes of hands-on time for purification of high-quality DNA.
- Accurate: Unbiased cellular lysis for accurate analysis of the microbial profile.
Original Manufacturer
Innovated in California, Made in the USA
Satisfaction 100% guaranteed, read Our Promise
Highlights
- Depletes Host and Enriches for Bacteria: Increase the ratio of bacteria to host DNA.
- Fast: Only 30 minutes of hands-on time for purification of high-quality DNA.
- Accurate: Unbiased cellular lysis for accurate analysis of the microbial profile.
Original Manufacturer
Innovated in California, Made in the USA
Satisfaction 100% guaranteed, read Our Promise
| Cat # | Name | Size | Price | Quantity |
|---|
| Cat # | Name | Size | Price | |
|---|---|---|---|---|
| D4300-4-60 | ZymoBIOMICS DNA Wash Buffer 2 | 60 ml | $37.40 | |
| D4300-1-40 | ZymoBIOMICS Lysis Solution | 40 ml | $46.70 | |
| D4300-2-100 | ZymoBIOMICS DNA Binding Buffer | 100 ml | $161.10 | |
| D4300-3-50 | ZymoBIOMICS DNA Wash Buffer 1 | 50 ml | $53.70 | |
| D4302-5-50 | ZymoBIOMICS DNase/RNase Free Water | 50 ml | $114.30 | |
| D4302-5-10 | ZymoBIOMICS DNase/RNase Free Water | 10 ml | $32.70 | |
| D3001-2-5 | Proteinase K w/ Storage Buffer Set | 5 mg | $24.50 | |
| D3001-2-20 | Proteinase K w/ Storage Buffer Set | 20 mg | $51.40 | |
| R1200-125 | DNA/RNA Shield (2X Concentrate) | 125 ml | $255.60 | |
| R1200-25 | DNA/RNA Shield (2X Concentrate) | 25 ml | $72.10 | |
| C1001-50 | Collection Tubes | 50 Pack | $17.50 | |
| C1001-500 | Collection Tubes | 500 Pack | $60.70 | |
| C1001-1000 | Collection Tubes | 1000 Pack | $105.10 | |
| D4310-1-20 | Host Depletion Solution | 20 ml | $35.00 | |
| D4310-2-5 | Microbial Selection Buffer | 5 ml | $29.10 | |
| D4310-3-50 | Microbial Selection Enzyme | 50 µl | $140.10 | |
| S6012-50 | ZR BashingBead Lysis Tubes (0.1 & 0.5 mm) | 50 Tubes | $117.80 | |
Description
Performance
Technical Specifications
| DNA Purity | High-quality DNA is eluted with ZymoBIOMICS® DNase/RNase Free Water and is suitable for all downstream applications including PCR and Next-Generation Sequencing. |
|---|---|
| DNA Recovery | Greater than 85% of total bacterial DNA is effectively recovered with greater than 90% of eukaryotic host DNA depleted. |
| Processing Time | Bacterial DNA is isolated from a single sample in less than 90 minutes. |
| Required Equipment | Microcentrifuge, vortex, tube rotator, and high-speed cell disruptor (recommended). |
| Sample Input | Up to 200 µl liquid sample. |
| Sample Type | Saliva, swabs, and bodily fluids from eukaryotic hosts with intact (living) bacteria cells. |
Resources
Documents
FAQ
Studies where shotgun metagenomic sequencing will be used to produce the microbial profile of a sample high in host DNA. To reduce the number of reads that will go to the host DNA, host DNA must first be depleted.
Feces have not been validated. Stool from a healthy donor is not expected to contain a high abundance of host DNA. However, if the stool is from a donor with a bowel related illness (e.g. ulcerative colitis), then host depletion may be useful.
Samples such as saliva, swabs, and bodily fluids from eukaryotic hosts with intact bacteria cells are directly compatible with this kit. Pre-treatment is required for whole blood and solid tissues. Samples in DNA/RNA Shield are not compatible.
No, the HostZero kit cannot be used to deplete host DNA from viral samples. Viral DNA will be removed with host DNA during the depletion step. The kit can only be used for host depletion from microbial samples.
Citations
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