HostZERO Microbial DNA Kit
D4310
HostZERO Microbial DNA Kit
Cat # | Name | Size | Price | Quantity |
---|
Documents

Highlights
- Depletes Host and Enriches for Bacteria: Increase the ratio of bacteria to host DNA.
- Fast: Only 30 minutes of hands-on time for purification of high-quality DNA.
- Accurate: Unbiased cellular lysis for accurate analysis of the microbial profile.
Description
DNA Purity | High-quality DNA is eluted with ZymoBIOMICS® DNase/RNase Free Water and is suitable for all downstream applications including PCR and Next-Generation Sequencing. |
---|---|
DNA Recovery | Greater than 85% of total bacterial DNA is effectively recovered with greater than 90% of eukaryotic host DNA depleted. |
Processing Time | Bacterial DNA is isolated from a single sample in less than 90 minutes. |
Required Equipment | Microcentrifuge, vortex, tube rotator, and high-speed cell disruptor (recommended). |
Sample Input | Up to 200 µl liquid sample. |
Sample Type | Saliva, swabs, and bodily fluids from eukaryotic hosts with intact (living) bacteria cells. |
Supplemental Info |
Q1: When should I use this kit?
Studies where shotgun metagenomic sequencing will be used to produce the microbial profile of a sample high in host DNA. To reduce the number of reads that will go to the host DNA, host DNA must first be depleted.
Q2: Is this kit compatible with feces samples?
Feces have not been validated. Stool from a healthy donor is not expected to contain a high abundance of host DNA. However, if the stool is from a donor with a bowel related illness (e.g. ulcerative colitis), then host depletion may be useful.
Q3: What sample types are compatible with this kit?
Saliva, throat swabs, sputum, bronchoalveolar lavage (BAL).
Samples that are not compatible: Blood, solid tissues and samples in DNA/RNA Shield are not compatible.
Cat # | Name | Size | Price | |
---|---|---|---|---|
D4300-4-60 | ZymoBIOMICS DNA Wash Buffer 2 | 60 ml | $35.20 | |
D4300-1-40 | ZymoBIOMICS Lysis Solution | 40 ml | $44.00 | |
D4300-2-100 | ZymoBIOMICS DNA Binding Buffer | 100 ml | $151.80 | |
D4300-3-50 | ZymoBIOMICS DNA Wash Buffer 1 | 50 ml | $50.60 | |
D4302-5-50 | ZymoBIOMICS DNase/RNase Free Water | 50 ml | $107.80 | |
D4302-5-10 | ZymoBIOMICS DNase/RNase Free Water | 10 ml | $30.80 | |
D3001-2-5 | Proteinase K w/ Storage Buffer Set | 5 mg | $23.10 | |
D3001-2-20 | Proteinase K w/ Storage Buffer Set | 20 mg | $48.40 | |
R1200-125 | DNA/RNA Shield (2X Concentrate) | 125 ml | $241.00 | |
R1200-25 | DNA/RNA Shield (2X Concentrate) | 25 ml | $68.00 | |
C1001-50 | Collection Tubes | 50 Pack | $16.50 | |
C1001-500 | Collection Tubes | 500 Pack | $57.20 | |
C1001-1000 | Collection Tubes | 1000 Pack | $99.00 | |
D4310-1-20 | Host Depletion Solution | 20 ml | $33.00 | |
D4310-2-5 | Microbial Selection Buffer | 5 ml | $27.50 | |
D4310-3-50 | Microbial Selection Enzyme | 50 µl | $132.00 | |
S6012-50 | ZR BashingBead Lysis Tubes (0.1 & 0.5 mm) | 50 Tubes | $111.10 | |