HostZERO Microbial DNA Kit
HostZERO Microbial DNA Kit
- Depletes Host and Enriches for Bacteria: Increase the ratio of bacteria to host DNA.
- Fast: Only 30 minutes of hands-on time for purification of high-quality DNA.
- Accurate: Unbiased cellular lysis for accurate analysis of the microbial profile.
The HostZERO Microbial DNA Kit is designed to overcome the challenge of contaminating host nucleic acids in microbial samples. This kit uses a novel method to reduce the amount of contaminating host DNA by selectively lysing the eukaryotic cells and degrading this DNA prior to total DNA purification. Paired with Zymo Research’s non-biased purification technology, the HostZERO Microbial DNA Kit allows for the exclusive capture of DNA from living microbial cells in a biological sample. This new technology is able to reduce the presence of human DNA in a saliva sample from 65% (untreated sample) to less than 1% (treated sample). Concurrently, the depletion process utilizes the ZymoBIOMICS non-biased DNA isolation technology for accurate data representation. Finally, the HostZERO Microbial DNA Kit recovers the highest amount of bacterial DNA as compared to other methods. By removing the presence of host DNA and reducing bias in purification, the HostZERO Microbial DNA Kit produces the highest-quality data for microbial samples.
|DNA Purity||High-quality DNA is eluted with ZymoBIOMICS® DNase/RNase Free Water and is suitable for all downstream applications including PCR and Next-Generation Sequencing.|
|DNA Recovery||Greater than 85% of total bacterial DNA is effectively recovered with greater than 90% of eukaryotic host DNA depleted.|
|Processing Time||Bacterial DNA is isolated from a single sample in less than 90 minutes.|
|Required Equipment||Microcentrifuge, vortex, tube rotator, and high-speed cell disruptor (recommended).|
|Sample Input||Up to 200 µl liquid sample.|
|Sample Type||Saliva, swabs, and bodily fluids from eukaryotic hosts with intact (living) bacteria cells.|
Q1: When should I use this kit?
Studies where shotgun metagenomic sequencing will be used to produce the microbial profile of a sample high in host DNA. To reduce the number of reads that will go to the host DNA, host DNA must first be depleted.
Q2: Is this kit compatible with feces samples?
Feces have not been validated. Stool from a healthy donor is not expected to contain a high abundance of host DNA. However, if the stool is from a donor with a bowel related illness (e.g. ulcerative colitis), then host depletion may be useful.
Q3: What sample types are compatible with this kit?
Saliva, throat swabs, sputum, bronchoalveolar lavage (BAL).
Samples that are not compatible: Blood, solid tissues and samples in DNA/RNA Shield are not compatible.
|D4300-4-60||ZymoBIOMICS DNA Wash Buffer 2||60 ml||$35.20|
|D4300-1-40||ZymoBIOMICS Lysis Solution||40 ml||$44.00|
|D4300-2-100||ZymoBIOMICS DNA Binding Buffer||100 ml||$151.80|
|D4300-3-50||ZymoBIOMICS DNA Wash Buffer 1||50 ml||$50.60|
|D4302-5-50||ZymoBIOMICS DNase/RNase Free Water||50 ml||$107.80|
|D4302-5-10||ZymoBIOMICS DNase/RNase Free Water||10 ml||$30.80|
|D3001-2-5||Proteinase K w/ Storage Buffer Set||5 mg||$23.10|
|D3001-2-20||Proteinase K w/ Storage Buffer Set||20 mg||$48.40|
|R1200-125||DNA/RNA Shield (2X Concentrate)||125 ml||$241.00|
|R1200-25||DNA/RNA Shield (2X Concentrate)||25 ml||$68.00|
|C1001-50||Collection Tubes||50 Pack||$16.50|
|C1001-500||Collection Tubes||500 Pack||$57.20|
|C1001-1000||Collection Tubes||1000 Pack||$99.00|
|D4310-1-20||Host Depletion Solution||20 ml||$33.00|
|D4310-2-5||Microbial Selection Buffer||5 ml||$27.50|
|D4310-3-50||Microbial Selection Enzyme||50 µl||$132.00|
|S6012-50||ZR BashingBead Lysis Tubes (0.1 & 0.5 mm)||50 Tubes||$111.10|