Tracking DNA Methylation in Breast Cancer at the Single-Cell and Spatial Levels

Single-cell DNA methylation sequencing is transforming how we read tumor epigenomes. This webinar covers practical and analytical approaches for profiling methylation heterogeneity at single-cell resolution in hormone-driven breast cancer. We also explore the role of 'epimutations' and how they can be used to reconstruct lineage trees.

• How single-cell methylation profiling scales to thousands of cells, and what sample inputs and preparation it requires
• Analytical strategies for calling methylation states, defining cell populations, and integrating methylation with transcriptome and chromatin readouts
• How methylation heterogeneity tracks endocrine response, tumor evolution, and early transformation in breast cancer

Rewiring of regulatory elements is a key hallmark of breast cancer initiation and progression