E2016 / E2017
- Quick and simple procedure for completely degrading DNA into its individual nucleotide component for quantitative analysis (e.g., whole-genome methylation analysis by HPLC, TLC, etc.).
- 1 hour, single-enzyme digest vs. conventional 6 - 16-hour multi-step enzyme digestion protocols
DNA Degradase from Zymo Research is a nuclease mix that quickly and efficiently degrades DNA to its individual nucleotide components. DNA Degradase is ideal for whole-genome DNA methylation analysis by many downstream applications (i.e., HPLC, TLC, etc.). Digestion with the enzyme is performed via a one-step procedure that is faster and simpler than other available methods.
|Assay Condition||DNA Degradase in 1X DNA Degradase Reaction Buffer. Incubate reaction mixtures at 37C for ≥ 1 hour.|
|Enzyme Inactivation||70C for 20 minutes|
|Storage||Store at -20C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage is at ≤ -70C.|
|Unit Definition||One unit (U) is the amount of enzyme required to degrade 1 µg of λ|