Successfully Added to Cart
Updated Cart Total:
0
items
Subtotal:
$0.00 USD
Customers also bought...
-
EZ DNA Methylation-Gold Kit (200 Rxns)Cat#: D5006The EZ DNA Methylation-Gold Kit is a refinement of our popular EZ DNA Methylation kit and uses heat denaturation instead of chemical denaturation of the... -
Quick-DNA Fungal/Bacterial Miniprep Kit (50 Preps)Cat#: D6005The Quick-DNA Fungal/Bacterial Miniprep Kit is designed for the simple and rapid isolation of DNA from tough-to-lyse fungi, including A. fumigatus, C. albicans, N. crassa... -
Human HCT116 DKO Non-Methylated DNA (5 µg/20 µl)Cat#: D5014-1The Human Methylated & Non-methylated DNA Set consists of two DNA controls (a CpG-methylated human DNA standard and a non-methylated human DNA standard), with a...
E. coli Non-Methylated Genomic DNA
Original Manufacturer
Innovated in California, Made in the USA
Satisfaction 100% guaranteed, read Our Promise
E. coli Non-Methylated Genomic DNA
| Cat # | Name | Size | Price | Quantity |
|---|---|---|---|---|
| D5016 | E. coli Non-Methylated Genomic DNA | 5 µg/20 µl | $119.90 |
-
+
|
Highlights
- Negative control for epigenetic experiments requiring DNA containing zero background levels of cytosine and adenosine methylation. Substrate for DNA methylation-sensitive restriction enzyme digestions.
Documents
Protocol: 
SDS (MSDS):
Supplemental Info
Product Description
Technical Specifications
| Concentration | 250 ng/µl in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0) |
|---|---|
| Genotype | ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10)TetS endA1 rpsL136 dam13::Tn9 xylA-5 mtl-1 thi-1 mcrB1 hsdR2 |
| Storage | ≤ -20°C |
Resources
Q1: How do you use these standards?
During NGS library prep, this standard can be spiked in to or run in parallel with experimental samples to monitor bisulfite conversion efficiency and/or workflow performance.
Q2: What reference genome should I use for alignment?
The reference genome of E. coli strain K-12 substrain MG1655 can be used for alignment and analysis. It can be accessed here.
