EZ RNA Methylation Kit
R5001 / R5002
- Specifically optimized for complete conversion of non-methylated cytosines in RNA.
- Ideal for all RNA inputs.
- Complete conversion of RNA in as little as 1 hour.
|Elution Volume||≥ 10 µl|
|Equipment||Thermocycler with heated lid and microcentrifuge.|
|Input||0.5 - 1 µg of RNA.|
|Processing Time||50 Minutes|
|Purity||Purified, converted RNA is of high quality and is well suited for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.|
Researchers from Europe showed that reduced levels of conserved RNA methyltransferase NSUN5 increase the lifespan and stress resistance in yeast, worms, and flies. They used the EZ RNA Methylation Kit to show that the methylation at C2278 of 25S ribosomal RNA in yeast, worms, and flies is highly conserved, suggesting that it is important for core ribosomal function. They also observed better reproducibility and higher conversion rate using Zymo Research’s EZ RNA Methylation Kit.Schosserer, M. et. al. (2015) Methylation of ribosomal RNA by NSUN5 is a conserved mechanism modulating organismal lifespan. Nat Commun. 6, 6158.
Researchers used the EZ RNA Methylation Kit from Zymo Research to investigate the methylation status of several important non-coding RNAs, including XIST and HOTAIR. They found that both XIST and HOTAIR contained the 5-methylcytosine modification, suggesting that RNA methylation may play an important role in regulating the activity of these RNA molecules.Amort T, Soulière MF, Wille A, Jia XY, Fiegl H, Wörle H, Micura R, & Lusser A (2013). Long non-coding RNAs as targets for cytosine methylation. RNA biology, 10 (6)