EZ RNA Methylation Kit

R5001 / R5002


EZ RNA Methylation Kit

R5001 / R5002

Cat # Name Size Price Quantity
R5001 EZ RNA Methylation Kit 50 Prep $189.00
+ -
R5002 EZ RNA Methylation Kit 200 Prep $599.00
+ -

Documents


Fast and reliable bisulfite conversion of RNA for methylation analysis.

Highlights


  • Specifically optimized for complete conversion of non-methylated cytosines in RNA.
  • Ideal for all RNA inputs.
  • Complete conversion of RNA in as little as 1 hour.

Description


The EZ RNA Methylation Kit features rapid and reliable bisulfite conversion of cytosines in RNA for methylation analysis. The kit streamlines the three-step process for complete conversion of cytosine in into uracil. RNA denaturation and bisulfite conversion processes are combined into a single step. No buffer preparation is necessary. The RNA Conversion Reagent is provided ready-to-use: simply add the reagent to an RNA sample and incubate as indicated. The innovative in-column desulphonation technology eliminates messy precipitation steps, ensuring researchers obtain consistent results. The product has been designed to minimize template degradation, loss of RNA during treatment and clean-up, and to provide complete conversion of cytosine for accurate methylation analysis. Recovered RNA is ideal for RT-PCR, sequencing, library preparation and Next-Gen sequencing.

Technical Specifications


Conversion > 99%
Elution Volume ≥ 10 µl
Equipment Thermocycler with heated lid and microcentrifuge.
Input 0.5 - 1 µg of RNA.
Processing Time 50 Minutes
Purity Purified, converted RNA is of high quality and is well suited for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.
Recovery > 80%

Citations


Kit Components