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EZ RNA Methylation Kit
R5001 / R5002
EZ RNA Methylation Kit
- Specifically optimized for complete conversion of non-methylated cytosines in RNA.
- Ideal for all RNA inputs.
- Complete conversion of RNA in as little as 1 hour.
The EZ RNA Methylation Kit features rapid and reliable bisulfite conversion of cytosines in RNA for methylation analysis. The kit streamlines the three-step process for complete conversion of cytosine in into uracil. RNA denaturation and bisulfite conversion processes are combined into a single step. No buffer preparation is necessary. The RNA Conversion Reagent is provided ready-to-use: simply add the reagent to an RNA sample and incubate as indicated. The innovative in-column desulphonation technology eliminates messy precipitation steps, ensuring researchers obtain consistent results. The product has been designed to minimize template degradation, loss of RNA during treatment and clean-up, and to provide complete conversion of cytosine for accurate methylation analysis. Recovered RNA is ideal for RT-PCR, sequencing, library preparation and Next-Gen sequencing.
|Elution Volume||≥ 10 µl|
|Equipment||Thermocycler with heated lid and microcentrifuge.|
|Input||0.5 - 1 µg of RNA.|
|Processing Time||50 Minutes|
|Purity||Purified, converted RNA is of high quality and is well suited for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.|
|W1001-1||DNase/RNase-Free Water||1 ml||$11.00|
|W1001-4||DNase/RNase-Free Water||4 ml||$13.20|
|R5001-1-1||RNA Conversion Reagent||1.5 ml||$24.00|
|R5001-3-10||RNA Desulphonation Buffer||10 ml||$17.00|
|R5001-3-40||RNA Desulphonation Buffer||40 ml||$45.00|
|C1004-50||Zymo-Spin IC Columns||50 Pack||$58.30|
|C1001-50||Collection Tubes||50 Pack||$16.50|
|R1013-2-25||RNA Binding Buffer||25 ml||$53.90|
|R1003-3-12||RNA Wash Buffer||12 ml||$33.00|
|R1003-3-24||RNA Wash Buffer||24 ml||$64.90|
|R1013-2-100||RNA Binding Buffer||100 ml||$140.80|