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    EZ RNA Methylation Kit

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    EZ RNA Methylation Kit

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    Cat # Name Size Price Quantity
    R5001 EZ RNA Methylation Kit 50 Prep $200.50
    - +
    R5002 EZ RNA Methylation Kit 200 Prep $635.50
    - +

    Highlights

    • Specifically optimized for complete conversion of non-methylated cytosines in RNA.
    • Ideal for all RNA inputs.
    • Complete conversion of RNA in as little as 1 hour.
    Description & Documents Specifications Components Citations Related Products

    Documents


    Product Description


    The EZ RNA Methylation Kit features rapid and reliable bisulfite conversion of cytosines in RNA for methylation analysis. The kit streamlines the three-step process for complete conversion of cytosine in into uracil. RNA denaturation and bisulfite conversion processes are combined into a single step. No buffer preparation is necessary. The RNA Conversion Reagent is provided ready-to-use: simply add the reagent to an RNA sample and incubate as indicated. The innovative in-column desulphonation technology eliminates messy precipitation steps, ensuring researchers obtain consistent results. The product has been designed to minimize template degradation, loss of RNA during treatment and clean-up, and to provide complete conversion of cytosine for accurate methylation analysis. Recovered RNA is ideal for RT-PCR, sequencing, library preparation and Next-Gen sequencing.

    Documents

    Technical Specifications

    Conversion > 99%
    Elution Volume ≥ 10 µl
    Equipment Thermocycler with heated lid and microcentrifuge.
    Input 0.5 - 1 µg of RNA.
    Processing Time 50 Minutes
    Purity Purified, converted RNA is of high quality and is well suited for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.
    Recovery > 80%

    Resources


    Cat # Name Size Price
    W1001-1 DNase/RNase-Free Water 1 ml $11.60
    W1001-4 DNase/RNase-Free Water 4 ml $14.00
    R5001-1-1 RNA Conversion Reagent 1.5 ml $25.40
    R5001-3-10 RNA Desulphonation Buffer 10 ml $18.00
    R5001-3-40 RNA Desulphonation Buffer 40 ml $47.80
    C1004-50 Zymo-Spin IC Columns 50 Pack $61.80
    C1001-50 Collection Tubes 50 Pack $17.50
    R1013-2-25 RNA Binding Buffer 25 ml $57.20
    R1003-3-12 RNA Wash Buffer 12 ml $35.00
    R1003-3-24 RNA Wash Buffer 24 ml $68.80
    R1013-2-100 RNA Binding Buffer 100 ml $149.40

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