MBP-Spin Protein Miniprep Kit

P2006 / P2007


MBP-Spin Protein Miniprep Kit

P2006 / P2007

Cat # Name Size Price Quantity
P2006 MBP-Spin Protein Miniprep Kit 10 Preps $82.00
+ -
P2007 MBP-Spin Protein Miniprep Kit 50 Preps $303.00
+ -

Documents


Fast protocol to purify MBP-tagged proteins from cell-free extracts.

Highlights


  • Fast: Purify MBP-tagged proteins from cell lysates in less than 6 minutes.
  • Simple: Prepare pure protein for small-scale studies using a spin-column.
  • Convenient: No special instrumentation needed other than a benchtop microcentrifuge.

Description


The MBP-Spin Protein Miniprep Kit provides a fast spin-column based purification technology for MBP-tagged proteins. Up to 1000 µg of MBP-tagged protein can be purified in 6 minutes and eluted in MBP-Elution Buffer. The purified protein is ultra-pure and can be used directly for enzymatic assays, biochemical analyses, SDS-PAGE and other sensitive applications. The straightforward spin, wash, and elute protocol dramatically simplifies protein purification and allows the end user to get results in minutes, not hours.

Technical Specifications


Affinity Matrix Amylose Resin
Binding Capacity 100 µl of Amylose Resin typically binds ≥ 400 µg of fusion protein.
Elution Method Excess Maltose
Elution Volume  ≥ 200 µl
Principle of Technology The MBP-Spin Protein Miniprep Kit uses an affinity matrix composed of amylose resin to specifically bind proteins fused to maltose-binding protein (MBP). MBP is known to significantly enhance the solubility of many proteins when fused to them.
Processing Time 6 minutes
Product Storage Please store the Amylose Resin at 4 °C. The other components can be stored at room temperature.
Purity Electrophoretically pure and suitable for enzyme kinetics, biochemical analyses, SDS-PAGE, and other applications.
Required Equipment Microcentrifuge
Sample Type Cell lysates or other complex protein mixtures containing MBP-tagged proteins.

Product FAQ


Q1: Can you use your own Amylose powder resuspended in 20 % ethanol instead of the supplied Amylose resin?

Q2: Which chemicals/reagents are compatible with the kit?

Q3: Which pH should be used with the kit?

Q4: Can the sample contain maltose?

Q5: How do you improve protein quality/purity?

Q6: How do you increase protein yield?

Q7: What might reduce protein yields?

Q8: Is there a difference in binding between MBP and the one containing mutations (published from NEB)?

Q9: What is the minimum elution volume that can be used?

Q10: Is there a recommended lysis protocol?

Q11: Is it possible to use lower speeds than the recommended 13,000 x g for centrifugation?

Q12: Is it necessary to equilibrate the resin?

Q13: Is there a difference in performance between Aymlose HF and Amylose resin?

Q14: Will eluting 4 times with 50 µl of elution buffer provide higher yields compared to eluting once with 200 µl?

Kit Components