MBP-Spin Protein Miniprep Kit
P2006 / P2007
- Fast: Purify MBP-tagged proteins from cell lysates in less than 6 minutes.
- Simple: Prepare pure protein for small-scale studies using a spin-column.
- Convenient: No special instrumentation needed other than a benchtop microcentrifuge.
The MBP-Spin Protein Miniprep Kit provides a fast spin-column based purification technology for MBP-tagged proteins. Up to 1000 µg of MBP-tagged protein can be purified in 6 minutes and eluted in MBP-Elution Buffer. The purified protein is ultra-pure and can be used directly for enzymatic assays, biochemical analyses, SDS-PAGE and other sensitive applications. The straightforward spin, wash, and elute protocol dramatically simplifies protein purification and allows the end user to get results in minutes, not hours.
|Affinity Matrix||Amylose Resin|
|Binding Capacity||100 µl of Amylose Resin typically binds ≥ 400 µg of fusion protein.|
|Elution Method||Excess Maltose|
|Elution Volume||≥ 200 µl|
|Principle of Technology||The MBP-Spin Protein Miniprep Kit uses an affinity matrix composed of amylose resin to specifically bind proteins fused to maltose-binding protein (MBP). MBP is known to significantly enhance the solubility of many proteins when fused to them.|
|Processing Time||6 minutes|
|Product Storage||Please store the Amylose Resin at 4 °C. The other components can be stored at room temperature.|
|Purity||Electrophoretically pure and suitable for enzyme kinetics, biochemical analyses, SDS-PAGE, and other applications.|
|Sample Type||Cell lysates or other complex protein mixtures containing MBP-tagged proteins.|
Q1: Can you use your own Amylose powder resuspended in 20 % ethanol instead of the supplied Amylose resin?
Q2: Which chemicals/reagents are compatible with the kit?
Q3: Which pH should be used with the kit?
Q4: Can the sample contain maltose?
Q5: How do you improve protein quality/purity?
Q6: How do you increase protein yield?
Q7: What might reduce protein yields?
Q8: Is there a difference in binding between MBP and the one containing mutations (published from NEB)?
Q9: What is the minimum elution volume that can be used?
Q10: Is there a recommended lysis protocol?
Q11: Is it possible to use lower speeds than the recommended 13,000 x g for centrifugation?
Q12: Is it necessary to equilibrate the resin?
Q13: Is there a difference in performance between Aymlose HF and Amylose resin?
Q14: Will eluting 4 times with 50 µl of elution buffer provide higher yields compared to eluting once with 200 µl?
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