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    PolStar NGS Library Prep qPCR Premix (2X)

    Hot start, high-fidelity, NGS-optimized polymerase packaged in a ready-to-use qPCR Premix. Streamline your NGS library prep workflow with qPCR capability!

    Highlights

    • High fidelity, hot-start NGS polymerase with minimal GC-bias across diverse templates.
    • Strong fluorescent signal for real-time quantification and auto-normalization of libraries in iconPCR® workflows.
    • Rapid reaction setup: just add water, primers, and template.
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    Highlights

    • High fidelity, hot-start NGS polymerase with minimal GC-bias across diverse templates.
    • Strong fluorescent signal for real-time quantification and auto-normalization of libraries in iconPCR® workflows.
    • Rapid reaction setup: just add water, primers, and template.
    Zymo Research's 100% Satisfaction Guarantee

    Original Manufacturer

    100% satisfaction guaranteed, read Our Promise

    Innovated in California, Made in the USA

    Cat # Name Size Price Quantity
    E2070 PolStar NGS Library Prep qPCR Premix 50 Rxns (1.25 ml) $135.00
    - +
    E2071 PolStar NGS Library Prep qPCR Premix 200 Rxns (5 ml) $486.00
    - +

    Description

    The PolStar™ NGS Library Prep qPCR Premix produces auto-normalized libraries when paired with qPCR instruments that control PCR cycling of individual reactions based on fluorescence (e.g. iconPCR®). The PolStar™ Premix uses a high fidelity, hot-start DNA polymerase to amplify diverse templates of varying sizes with minimal bias making it an ideal choice for a variety of NGS applications including Illumina WGS & full-length 16S rRNA gene sequencing. The included SYTO9® fluorescent dye enables simultaneous amplification and quantification of high quality NGS libraries while the ready-to-use formulation minimizes hands-on time and handling errors.

    Performance

    Technical Specifications
    Activity: The polymerase contains both 5'-3' polymerization and 3'-5' proofreading activity. Both activities are inhibited prior to initialization.
    Concentration: 2X
    Storage: Store at ≤ -20°C for up to 12 months. Minimize exposure to light. Avoid repeated freeze/thawing of reagents.
    Equipment: Real-time PCR can be performed on any instrument that has optical scanners that do not require a passive reference dye. Instruments that individually modulate sample cycling via fluorescence (e.g. iconPCR®) can produce auto-normalized products.

    Resources

    Documents

    FAQ

    The PolStarTM NGS Library Prep qPCR Premix can accommodate an amplicon length of 2kb with the cycling conditions provided in the protocol. Robust amplification of templates up to or exceeding 10kb can be achieved but may require empirical determination of cycling conditions for optimal results.

    When combined with qPCR instruments that control PCR cycles of individual samples based on fluorescence (e.g. iconPCR®), the PolStarTM Premix can produce auto-normalized libraries while enabling real-time QC during library amplification.

    2.2-3.6x10-6 errors/bp incorporated.

    Yes. Both the polymerization and proofreading activities of the polymerase are strongly inhibited prior to heat-activation.

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