Proteinase K w/ Storage Buffer Set

D3001-2-5 / D3001-2-20 / D3001-2-60 / D3001-2-125


Proteinase K w/ Storage Buffer Set

Cat # Name Size Price Quantity
D3001-2-5 Proteinase K w/ Storage Buffer Set 5 mg $23.10
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D3001-2-20 Proteinase K w/ Storage Buffer Set 20 mg $48.40
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D3001-2-60 Proteinase K w/ Storage Buffer Set 60 mg $145.20
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D3001-2-125 Proteinase K w/ Storage Buffer Set 125 mg $249.70
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Proteinase K w/ Storage Buffer Set

Highlights
  • Lyophilized enzyme provided with Proteinase K Storage Buffer.
Description

Proteinase K is a stable serine protease with broad substrate specificity and will degrade many proteins in their native conformation even in the presence of detergents (e.g., SDS). The enzyme is frequently used in molecular biology applications to digest unwanted proteins such as nucleases from DNA and/or RNA preparations from microorganisms, cells, and plants.

Enzyme Commission Number (EC 3.4.21.64)
pH and Temperature Range 4.0 to 12.0 (8.0 is optimum), 25 to 65°C
Source Engyodontium album
Specific Activity > 30 units/mg protein
Supplemental Info
Unit Definition One unit (U) of enzyme will hydrolyze urea-denatured hemoglobin to produce 1.0 µmole of tyrosine per minute at pH 7.5 at 37°C.

Size Add Proteinase K Storage Buffer
5 mg 260 µl
20 mg 1040 µl
60 mg 3120 µl
125 mg 6500 µl
Reconstitute lyophilized Proteinase K at 20 mg/ml with Proteinase K Storage Buffer. Table provides recommended volumes for different sample sizes (mg); Concentration can be adjusted as needed.