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    Quick-16S Plus NGS Library Prep Kit (V1-V2) Sample

    The Quick-16S/ITS™ NGS Library Prep Sampler provides 8 reactions from each of our Quick-16S/ITS™ Plus library prep kits as well as our Quick-16S™ Full-Length Library Prep Kit. Sample the entire Microbiomics library prep portfolio in a single, convenient format.

    Highlights

    • The included Quick -16S/ITS™ Plus reactions produce automatically normalized libraries, regardless of sample input, and target a variety of bacterial 16S gene regions in addition to the fungal ITS2 region.
    • The included Quick -16S™ Full-Length Library Prep Kit reactions offer increased taxonomic resolution for samples sequenced on PacBio® & Oxford Nanopore Technologies® long-read platforms.
    • All full-size versions of the kits offered as part of the sampler are 100% automation ready with only a single PCR step, and support multiplexing of 96, 384, or 768 samples.
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    Highlights

    • The included Quick -16S/ITS™ Plus reactions produce automatically normalized libraries, regardless of sample input, and target a variety of bacterial 16S gene regions in addition to the fungal ITS2 region.
    • The included Quick -16S™ Full-Length Library Prep Kit reactions offer increased taxonomic resolution for samples sequenced on PacBio® & Oxford Nanopore Technologies® long-read platforms.
    • All full-size versions of the kits offered as part of the sampler are 100% automation ready with only a single PCR step, and support multiplexing of 96, 384, or 768 samples.
    Zymo Research's 100% Satisfaction Guarantee

    Original Manufacturer

    Satisfaction 100% guaranteed, read Our Promise

    Innovated in California, Made in the USA

    Cat # Name Size Price Quantity

    Description

    The Quick-16S/ITS™ NGS Library Prep Sampler provides 8 reactions from each of our Quick -16S/ITS™ Plus library prep kits targeting the V1-V2, V1-V3, V3-V4, V4, and fungal ITS2 hypervariable regions. Additionally provided are 8 reactions of the Quick -16S™ Full-Length Library Prep Kit, designed for usage on PacBio® & Oxford Nanopore® long read platforms to provide users unparalleled taxonomic resolution. Users can now explore all Microbiomics library prep kit offerings in a single, convenient sampling format ideally suited to parallel evaluation of several target regions.

    Performance

    Technical Specifications
    Primer Sequences (adapters not shown) V3-V4: 341f(CCTACGGGDGGCWGCAG, CCTAYGGGGYGCWGCAG; 17 bp), 806r(GACTACNVGGGTMTCTAATCC; 24 bp)
    ITS: ITS3f (GCATCGATGAAGAACGCAGC; 20 bp), ITS4r (TCCTCCGCTTATTGATATGC; 20 bp)
    V4: 515f (GTGYCAGCMGCCGCGGTAA; 19 bp), 806r (GGACTACNVGGGTWTCTAAT; 20 bp)
    V1-V2: 27f (AGRGTTYGATYMTGGCTCAG; 20 bp), 341r (CTGCWGCCHCCCGTAGG; 17 bp)
    V1-V3: 27f (AGRGTTYGATYMTGGCTCAG; 20 bp), 515r (TBACCGCGGCTGCTGGCAC; 19 bp)
    Full-Length 16S: 27f (AGRGTTYGATYMTGGCTCAG; 20 bp), 1492r (RGYTACCTTGTTACGACTT; 19 bp)
    Amplicon Sizes V3-V4: ~606 bp
    ITS: ~480 bp
    V4: ~388 bp
    V1-V2: ~492 bp
    V1-V3: ~650 bp
    Full-Length 16S: ~1500 bp
    Barcode Sequences 10bp barcodes are used for all provided reactions. Barcode sequences are available for download in the 'Documents' section.
    Index Primers Unique dual indexes (barcodes) are used to label individual samples for all Quick-16S/ITSTM Plus reactions while combinational indexes are used for the Quick-16STM Full-Length Library Prep Kit.
    Required Equipment Microcentrifuge, plate spinner (centrifuge), 96-well real-time quantitative PCR system (SYBR Green compatible) or standard PCR system, and 96-well real-time PCR plates.
    Sample Input Purified microbial DNA ≤100 ng, free of PCR inhibitors.
    Sequencing Platform Sequencing of V1-V2, V1-V3, V3-V4, and fungal ITS2 libraries require an Illumina Miseq/NextSeq. Sequencing of V4 libraries is possible on all Illumina platforms (with the use of custom sequencing primers). Full-length 16S libraries are compatible with both PacBio & Oxford Nanopore instruments.

    Resources

    Documents

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