Quick-DNA Midiprep Plus Kit
- Extract high-quality DNA easily and reliably from any biological fluids, cultured/monolayer cells, or solid tissues.
- Zymo-Spin Technology ensures DNA is ready for all sensitive downstream applications such as qPCR, DNA-sequencing, arrays, and methylation analysis.
The Quick-DNA Plus Kits are the easiest DNA isolation kits for high yield total DNA extraction (e.g., genomic, mitochondrial, viral), cell culture, solid tissue, saliva, and any biological fluid sample. Innovative reagents and Zymo-Spin Technology allow for ultra-pure and concentrated genomic DNA > 50 kb to be eluted in as little as 10 µl. Zymo-Spin Columns ensure no buffer retention. Purified DNA is RNA-free bypassing the need for RNase A treatment and ensuring accurate quantification for applications like library preparations. Isolated DNA is suitable for immediate use in sensitive downstream applications including qPCR, DNA-seq, arrays, and methylation analysis.
|Elution Volume||≤ 200 µl|
|Equipment||Water bath or heat block (55°C), centrifuge or vacuum source and manifold, microcentrifuge, vortex, conical tubes (15 – 50 ml), and microcentrifuge tubes.|
|Purity||High quality DNA is ready for all sensitive downstream applications such as PCR, endonuclease digestion, Southern blotting, genotyping, Next-Gen sequencing, bisulfite conversion, etc. (A260/A230≥ 2.0).|
|Size Range||Capable of recovering genomic and mitochondrial DNA sized fragments > 50 kb. If present, parasitic, microbial, and viral DNA will also be recovered.|
|Workflow||Utilize a Proteinase K Digestion and Zymo-Spin Column for effective recovery of DNA.|
|Yield||The DNA binding capacity of the column is 125 µg. Typically, mammalian tissues yield: 1-3 µg DNA per mg skeletal, heart, lung, and brain tissues and 3-5 µg DNA per mg liver and kidney. Human whole blood will yield 3-7 µg DNA per 100 µl blood sampled.|
Q1: What is the difference between the digestion buffers? Can one buffer be used for all sample types?
Q2: Can Quick-DNA process crude lysates?
Q3: What is the purpose of adding beta-mercaptoethanol? Can this step be substituted or omitted?
Q4: Can I use Quick-DNA Plus to clean-up previously isolated DNA?
Q5: What are the expected yields for each sample type?
Q6: I’m seeing some yield inconsistencies with my blood samples, what’s happening?
Q7: Can Proteinase K digestion be performed overnight in DNA/RNA Shield?
Q8: What is the difference between Quick-DNA and Quick-DNA Plus kits?
Q9: Can the Quick-DNA Plus kit be used with bacterial samples?
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