Quick-RNA Tissue/Insect Kit
- High Quality: Extract intact high quality total RNA (including small/micro RNAs) from insect and arthropod specimens (mosquitoes, bees, lice, ticks, Drosophila melanogaster etc.)
- Homogenization: Ultra-high density BashingBead technology for complete disruption of tough specimen.
- Inhibitor-Free: RNA is ready for all downstream applications including sequencing, RT-PCR, microarray, etc.
The Quick-RNA Tissue/Insect Microprep Kit provides for rapid isolation of total RNA from various insect and arthropod specimens (e.g. mosquitoes, bees, lice, ticks, Drosophila melanogaster). The Quick-RNA Tissue/Insect Microprep Kit employs ultra-high density ZR BashingBeads for sample homogenization and a robust buffer system delivering total RNA (including small RNAs) as well as DNA removal from a variety of samples. The Zymo-Spin IIIC Column allows for high-capacity DNA elimination and the subsequent Zymo-Spin IC Column efficiently binds total RNA. The DNase/RNase-Free Water eluted RNA is suitable for subsequent procedures including RT-PCR.
|Equipment||Microcentrifuge, vortex and/or cell disrupter/pulverizer (optional)|
|Purity||RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.|
|Sample Source||Small amounts (n ≥ 1 and ≤ 10 mg) of insect and arthropod specimens (e.g. mosquitoes, bees, lice, ticks, Drosophila melanogaster)|
|Size Range||Total RNA ≥ 17 nt|
|Yield||10 µg RNA (binding capacity), ≥6 µl (elution volume)|