Strep-Spin Protein Miniprep Kit
P2004 / P2005
- Fast: Purify Strep-tagged proteins from cell lysates in less than 7 minutes.
- Simple: Prepare pure protein for small-scale studies using a spin-column.
- Convenient: No special instrumentation needed other than a benchtop microcentrifuge.
The Strep-Spin Protein Miniprep Kit™ provides a fast spin-column based purification technology for Strep-tagged proteins. Up to 450 µg of Strep-tagged protein can be purified in 7 minutes and eluted in Strep-Elution Buffer. The purified protein is ultra-pure and can be used directly for enzymatic assays, protein biochemical analyses, SDS-PAGE and other sensitive applications. The straightforward spin, wash, and elute protocol dramatically simplifies protein purification and allows the end user to get results in minutes, not hours.
|Affinity Matrix||Strep-Tactin® XT Superflow® 50%|
|Binding Capacity||100 µl of Strep-Tactin® XT Superflow® 50% suspension can bind 15 nmol biotin (approx. 450 µg of a 30 kDa Twin-Strep-tag® protein).|
|Elution Method||Biotin excess|
|Elution Volume||Recommended 3 x 200 µl|
|Principle of Technology||The Strep-Spin Protein Miniprep Kit is based on a novel StrepTactin® XT Superflow® resin which binds to Twin Strep-tag® with very high affinity. This tag contains two Strep-tag® II peptides connected via a Glycine/Serine linker sequence. Hence this kit is optimized for the purification of Twin-Step®-tagged proteins; however it will also work efficiently for proteins with single Strep-tags.|
|Processing Time||7 minutes|
|Product Storage||Please store the Strep-Tactin® XT Superflow® 50% suspension, Strep-Wash Buffer, and Strep-Elution Bufer at 4°C. The other components can be stored at room temperature.|
|Protein Purity||Electrophoretically pure. Purified high-quality protein is suitable for enzyme kinetics, protein biochemical analyses, SDS-PAGE, and other applications.|
|Sample Type||Cell lysates or other complex protein mixtures containing Strep-tagged proteins.|
Q1: Which Step-Tag amino acid sequences are recommended?
Q2: Can the kit be used to purify membrane proteins?
Q3: Can the sample contain biotin?
Q4: Which pH should be used with the kit?
Q5: Which chemicals/reagents are compatible with the kit?
Q6: Is there a recommended lysis protocol?
Q7: How do you improve protein quality/purity?
Q8: Can less then 200 µl of Strep-Spin Elution Buffer be used for the elution step?
Q9: Is it necessary to equilibrate the resin?
Q10: What might reduce protein yields?
Q11: How do you purify insoluble proteins?
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