Yeast Protein Kit

Y1002


Yeast Protein Kit

Cat # Name Size Price Quantity
Y1002 Yeast Protein Kit 200 Preps $83.60
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Documents


Highlights
  • Convenient: Rapid method for efficient lysis of yeast for downstream protein and DNA analyses.
  • Versatile: Procedure suitable for any fungal species susceptible to Zymolyase.
  • Effective Spheroplasting: Ideal protocol for Western blotting and PCR.
Description

The Yeast Protein Kit is a simple and convenient method for the rapid and thorough lysis of yeast cells. The kit has been optimized for use with S. cerevisiae and C. albicans but can be used for any fungal species that is susceptible to yeast lytic enzyme digestion. The digestion procedure effectively generates spheroplasts of yeast cells, making them ideal for both protein and DNA analyses including Western blotting and PCR, respectively.

Applicable For Spheroplasts are ideal for both protein and DNA analyses including Western blotting and PCR, respectively.
Equipment Microcentrifuge & Heat Block/Bath
Processing Time 35 Minutes
Sample Source Cell Culture (Colonies/Patches or Liquid Culture)

Any strains susceptible to Zymolyase, which includes the following fungal genera: Asbya Kloekera Candida Kluyveromyces Debaryomyces Lipomyces Eremothecium Metschikowia Endomyces Pichia Hansenula Pullularia Hanseniaspora Saccharomyces Saccaromycodes Saccharomycopsis Schizosaccahromyces Torulopsis

Cat # Name Size Price
Y1002-1-6 Y-Lysis Buffer 6 ml $17.60