ZR-96 Genomic DNA Clean & Concentrator-5 Kit
D4066 / D4067
- Quick, high-throughput (96-well) recovery of large-sized DNA from any enzymatic reaction or impure preparation without messy precipitations
- UniqueUnique spin column for low volume elution of ultra-pure, high-yield DNA. spin column for low volume elution of ultra-pure, high-yield DNA
- Eluted DNA is ideal for PCR, endonuclease digestion, sequencing, etc.
The ZR-96 Genomic DNA Clean & Concentrator (DCC) is a 96-well gDNA clean up kit that provides quick (5 minute) recovery of ultra-pure, large-sized DNA (e.g., genomic, mitochondrial, plasmid (BAC/PAC), viral, phage, (wga) DNA, etc.) from any enzymatic reaction or impure preparation (e.g., Proteinase K digestion). There is no need for organic denaturants, chloroform, or messy precipitations: simply add the specially formulated ChIP DNA Binding Buffer to a sample and then transfer the mixture to the supplied Zymo-Spin Column. Eluted DNA is suitable for sequencing, PCR, endonuclease digestion, and other enzymatic procedures. This 96-well gDNA clean up kit is also compatible with smaller DNAs (50 bp to 10 kb) from PCR, digestions, crude plasmid preparations, cDNA synthesis, etc.
|Applicable For||Eluted DNA is ideal for ligation, sequencing, labeling, PCR, microarray, transfection, transformation, restriction digestion procedures, and any other sensitive downstream application|
|Elution Volume||≥ 15 µl of DNA Elution Buffer|
|Equipment||Centrifuge w/ microplate carriers|
|Purity||A260/A280 > 1.8, A260/A230 > 1.8|
|Sample Source||Enzymatic reactions or impure preparations containing genomic DNA|
|Sample storage||Eluted DNA can be used immediately or stored at ≤ -20°C|
|Size Range||> 50 bp and up to 200 kb|
|Yield||Up to 5 µg DNA. Recovery of DNA ranges from 70 - 95%|
Q1: What is the lower limit and minimal amount of DNA that can be recovered?
Q2: How to process naked DNA stored in DNA/RNA Shield?
Q3: What to do if ethanol addition to the DNA Wash Buffer was omitted?
Q4: What happens if more DNA was loaded on the columns than the stated maximum binding capacity?
Q5: How many times can columns be reloaded?
Q6: What is the minimum input volume of DNA sample?
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