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Zymolyase
Yeast lytic enzyme for simple and effective digestion of fungal cell walls.
Highlights
- 100 T Equivalent: Prepared from Arthrobacter lutes. Essential enzyme activities are β-1,3-glucanase and β-1,3-glucan laminaripentao-hydrolase.
- Convenient: Provided lyophilized along with a storage buffer for reconstitution.
- Efficient Cell Wall Digestion: Supplied storage buffer has been optimized to confer maximum levels of enzymatic activity.
Original Manufacturer
Innovated in California, Made in the USA
Satisfaction 100% guaranteed, read Our Promise
Highlights
- 100 T Equivalent: Prepared from Arthrobacter lutes. Essential enzyme activities are β-1,3-glucanase and β-1,3-glucan laminaripentao-hydrolase.
- Convenient: Provided lyophilized along with a storage buffer for reconstitution.
- Efficient Cell Wall Digestion: Supplied storage buffer has been optimized to confer maximum levels of enzymatic activity.
Original Manufacturer
Innovated in California, Made in the USA
Satisfaction 100% guaranteed, read Our Promise
| Cat # | Name | Size | Price | Quantity |
|---|---|---|---|---|
| E1006 | R-Zymolyase (With RNase A) | 1000 U | $110.80 |
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| E1005 | Zymolyase | 2000 U | $148.20 |
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| E1004 | Zymolyase | 1000 U | $87.60 |
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Description
Performance
Technical Specifications
| Activity | β-1,3-glucan laminaripentao-hydrolase and β-1,3-glucanase (trace amount of protease, ca. 1.5 units per 10 µl). DNase and RNase: none detectable. |
|---|---|
| Concentration | 5 U/µl |
| Deactivation | Lytic activity is lost in 5 minutes at 60°C |
| Enzyme Source | Arthrobactor luteus |
| Format | Lyophilized enzyme provided w/ storage buffer |
| Optimum pH & Temp | pH 7.5, 35°C (lysis of viable yeast), pH 6.5, 45°C (hydrolysis of yeast glucan). |
| Product Storage | Zymolyase is stable for over 1 year at -20°C or many years below -70°C. |
| Protein Concentration | ~10-15 mg/ml |
| Susceptible Fungal Genera: | Asbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloekera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Saccharomyces, Saccharomyces, Saccharomycopsis, Schizosaccahromyces, Torulopsis |
| Unit Definition | One lytic unit is defined as catalyzing a 10% decrease in optical density at 800 nm (OD800) at 30°C in 30 minutes. |
| Usage | Spheroplast/Protoplast formation; Yeast cell fusion; Yeast transformation; Lysis of fungi |
Resources
FAQ
Zymolyase is very efficient, but here are some tips:
1. Use fresh and early log phase cells, which are more susceptible to the lytic properties of Zymolyase.
2. Using less cells as input can improve overall lysis efficiency.
3. Zymolyase digestion time can also be increased to 2 – 16 hours.
Citations
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