Zyppy-96 Plasmid MagPrep Kit

D4100 / D4101 / D4102


Zyppy-96 Plasmid MagPrep Kit

D4100 / D4101 / D4102

Cat # Name Size Price Quantity
D4100 Zyppy-96 Plasmid MagPrep Kit 2 x 96 Preps. $315.00
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D4101 Zyppy-96 Plasmid MagPrep Kit 4 x 96 Preps. $555.00
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D4102 Zyppy-96 Plasmid MagPrep Kit 8 x 96 Preps. $997.00
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Documents


App Note: PDF   |   App Note - Hamilton: PDF   |   Protocol: PDF   |   SDS (MSDS): PDF   |  
Pellet-free, high-quality plasmid DNA in only 8 minutes

Highlights


  • Pellet-Free: Rapid direct lysis procedure omits cell-pelleting and resuspension steps
  • High Quality: Ready for PCR, sequencing, cloning, and transfection
  • Ideal for Synthetic Biology: Fastest, high-throughput automated method for preparing high-quality plasmid DNA.

Description


The Zyppy-96 Plasmid MagBead Miniprep is the fastest high-throughput (96-well), Pellet-Free plasmid DNA kit available for efficient isolation of plasmid DNA from E. coli. This plasmid DNA kit features a modified alkaline lysis system that bypasses tedious centrifugation, pelleting, and re-suspension steps common to conventional procedures. Instead, the uniquely formulated Deep Blue Lysis Buffer is added directly to bacterial cultures in a 96-well block. Buffer neutralization and lysate separation steps are expedited using a specially designed Neutralization Buffer. Plasmid DNA is then purified using the featured magnetic bead based technology. This straightforward procedure is compatible for either automated or manual processing. Eluted plasmid DNA is of the highest quality, endotoxin-free, and is well suited for use in restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications including transfection. An overview of the purification procedure is shown below.

Technical Specifications


Applicable For Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA.
Elution Volume ≥ 30 µl
Equipment Magnetic Stand, Heating Element, or Liquid Handler
Processing Time 60 min
Purity Typical Abs 260/280 ≥1.8.
Size Range Up to 25 kb
Yield Up to 5 µg per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. Coli processed.

Product FAQ


Q1: What is the composition of the Zyppy Elution buffer?

Q2: Can the purified plasmid be transfected into eukaryotic cell lines?

Q3: Can the Zyppy Plasmid kits be used to isolate large constructs (BAC/PAC)?

Q4: What are the endotoxin levels in plasmid DNA isolated with the Zyppy Kits?

Q5: I ran out of Zyppy Wash Buffer. Can I substitute it with a homemade solution or Wash Buffer from another kit?

Reviews


“The speed I was doing a side by side comparison with Invitrogen and it is really nice to go from culture to plasmid DNA in less than 15 min.  Also the precipitate was really solid, no floaties, so it was very easy to pipet off the supernatant.”

- Rebecca N.

“It performs just as well as the Qiagen mini-prep kit (the accepted lab-standard).  The kit was very easy to follow and the resultant DNA was compatible with our complex downstream procedures.”

- Richard F.

"The results gave what they promised!  Clean DNA.">/em<

- Pamela R (UT Medicine San Antonio)

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Kit Components