The EZ DNA Methylation-Direct Kits are a further refinement of our popular EZ DNA Methylation and EZ DNA Methylation-Gold kits. These bisulfite conversion kits feature reliable and complete DNA bisulfite conversion directly from blood, tissue, and cells without the prerequisite for DNA purification. The increased sensitivity of these bisulfite conversion kits make it possible to amplify bisulfite-converted DNA from as few as 10 cells or 50 pg DNA. Like the EZ DNA Methylation-Gold kits, DNA denaturation and bisulfite conversion processes are combined into a single step. Recovered bisulfite-converted DNA from these bisulfite conversion kits is ideal for PCR amplification for downstream analyses including restriction endonuclease digestion, sequencing, microarrays, etc.
The EZ DNA Methylation-Direct Kits include the popular EZ-96 DNA Methylation-Direct MagPrep - a magnetic bead based bisulfite conversion kit that allows simple and reliable DNA bisulfite conversion directly from blood, tissue, and cells without the prerequisite for DNA purification. The increased sensitivity of this magnetic bead based bisulfite conversion kit makes it possible to amplify bisulfite converted DNA from as few as 10 cells or 50 pg DNA. These innovations have been coupled to a magnetic bead based clean-up for high-throughput methylation analysis. Desulphonation and clean-up of the converted DNA is performed while bound to the MagBinding Beads. This magnetic bead based bisulfite conversion kit has been designed to minimize template degradation, loss of DNA during treatment and clean-up, and to provide complete conversion of unmethylated cytosines. Recovered DNA is ideal for PCR, array, bisulfite and next-generation sequencing, etc.