EZ-96 DNA Methylation-Direct Kit

D5023 / D5022


EZ-96 DNA Methylation-Direct Kit

D5023 / D5022

Cat # Name Size Price Quantity
D5023 EZ-96 DNA Methylation-Direct Kit (Deep-well) 2 x 96 Rxns. $442.00
+ -
D5022 EZ-96 DNA Methylation-Direct Kit (Shallow- well) 2 x 96 Rxns. $442.00
+ -

Documents


A complete and reliable bisulfite conversion kit that eliminates cumbersome DNA precipitation steps and allows DNA bisulfite conversion directly from blood, tissue, and cells without prior DNA purification.

Highlights


  • High-throughput 96-well bisulfite conversion of DNA directly from blood, soft tissue, cells, FFPE samples, and LCM samples.
  • Compatible with small sample inputs as few as 10 cells or 50 pg DNA.
  • DNA recovered by this bisulfite conversion kit is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.

Description


The EZ-96 DNA Methylation-Direct Kit is a 96-well bisulfite conversion kit that features reliable and complete DNA bisulfite conversion directly from blood, tissue, and cells without the prerequisite for DNA purification. The increased sensitivity of this 96-well bisulfite conversion kit makes it possible to amplify bisulfite-converted DNA from as few as 10 cells or 50 pg DNA. Recovered bisulfite-converted DNA is ideal for PCR amplification for downstream analyses including restriction endonuclease digestion, sequencing, microarrays, etc.

Technical Specifications


Applications Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-generation sequencing, PCR amplification, etc.
Conversion > 99.5%
Elution Volume Shallow well: > 30 µl
Deep-well: >15 µl
Equipment Thermocycler with heated lid, swinging-bucket centrifuge with plate carriers.
Input Samples containing between 50 pg to 2 µg of DNA. For optimal results, the amount of input DNA should be from 200 to 500 ng. The number of cells per standard treatment can range from 10-105 cells.
Processing Time 4 hours
Recovery > 80%
Sample Source Blood, tissue, cells, FFPE, LCM-derived samples, purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc.

Product FAQ


Q1: Tips for bisulfite primer design?

Q2: Is incubation with Desulphonation Buffer for longer than 20 minutes recommended?

Q3: Does bisulfite conversion only occur in a CpG context?

Q4: Which polymerase is recommended for amplification from bisulfite converted DNA?

Q5: What is the minimum DNA size that can be recovered?

Q6: How to quantify / visualize converted DNA?

Q7: What leads to poor conversion efficiency/ low yields?

Q8: How long is bisulfite converted DNA stable at -20 °C?

Product Video


Citations


Kit Components