ZymoTaq qPCR Premix
E2054 / E2055
- Hot-start polymerase for easy setup at room temperature.
- Robust amplification of DNA for genotyping, SNP, and HRM analysis.
- Strong fluorescent signal for real-time and quantitative PCR assays.
|Activity||5' - 3' DNA polymerization|
|Concentration||Reaction conditions at 1X (50 µl total volume) will contain 2 U of ZymoTaq DNA polymerase.|
|Equipment||Real-time PCR can be performed on any instrument that has optical scanners that do not require passive reference dye.|
|Storage||Store at ≤ -20°C for up to 12 months. Minimize exposure to light. Avoid repeated freeze/thawing of reagents.|
|Unit Definition||One unit (U) enzyme of ZymoTaq DNA Polymerase is defined as the amount of enzyme required for the incorporation of 10 nmol dNTPs into an acid-insoluble form in 30 minutes at 72°C.|
Q1: What is the error rate of the ZymoTaq DNA polymerase?
Q2: Which is the maximal amplicon size generated using the ZymoTaq DNA polymerase?
Q3: What is the benefit of the ZymoTaq DNA PCR systems?
Q4: Does ZymoTaq DNA polymerase have a 5’ to 3’ exonuclease activity?
The ZymoTaq PreMix was specifically used to amplify bisulfite-treated DNA with no reports of inefficient or nonspecific amplification in a study of dynamic DNA methylation expression levels during different development stages. Results showed that timing of variations in methylation patterns were specific for genes related to growth.Fang X, et. al. (2013) Global and gene specific DNA methylation changes during zebrafish development. Comp Biochem Physiol B Biochem Mol Biol. 166(1):99-108.