Magnetic Bead-Based DNA Sizing and Concentration for Library Clean-Up

with Select-a-Size DNA Clean & Concentrator MagBead Kit

PROTOCOL

DATA SHEET

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High quality tunable DNA fragment

size selection 150 — 800 bps

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Rapid purification with

high reproducibility

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Automation friendly and

scalable protocol

High Quality Tunable Fragment Size Selection

Superior Fragment Size Selectivity

Graph showing tunable fragment size selection. — Select-a-Size DNA Clean & Concentrator Magbead Kit provides consistent performance in both short fragment (left-sided) depletion and large fragment (right-sided) depletion Size Selections. The workflow allows for specific cutoffs that can be modified to suit reaction clean-up, left-sided, right-sided or even double-sided size selection. The DNA fragment size selection range is 150 — 800 bp.

Tight Size Selection Capabilities

Select-a-Size DNA Clean & Concentrator MagBead Kit demonstrates tight size selection capabilities to remove discrete fragments while desired DNA fragments are unaffected by the selection process.

Graph showing left-sided selection of sonicated Salmon Sperm DNA — Select-a-Size DNA Clean & Concentrator MagBead Kit selection has a highly discernable resolution for incremental cut-offs. In this example, Select-a-Size magbeads are capable of tunable small fragment depletions in a range of bead ratios from 0.6X to 1.5X. The Select-a-size MagBead selection is able to remove the bulk of the unwanted material from an initial input sample, yet able to maintain a high recovery of the remaining desirable DNA.

Graph showing right-sided selection of sonicated Salmon Sperm DNA — Select-a-Size DNA Clean & Concentrator MagBead Kit selection has a highly discernable resolution for incremental cut-offs. In this example, Select-a-Size magbeads are capable of tunable small fragment depletions in a range of bead ratios from 0.6X to 1.5X. The Select-a-size MagBead selection is able to remove the bulk of the unwanted material from an initial input sample, yet able to maintain a high recovery of the remaining desirable DNA.

Increased Confidence with Highly Reproducible Fragment Size Selections

Precise Low Volume Magnetic Bead Transfer

Image of Select-a-Size DNA Clean & Concentrator Magbead

Image of Conventional Fragment Selection Magnetic Beads

Reduced fluid surface adhesion for improved microliter pipetting. More consistent low volume magnetic bead transfers due to less reagent “stickiness” to pipet tips.

High Reproducibility and Repeatability

Bar chart showing Small Fragment Depletion Yield Repeatability

Fragment size selections are highly reproducible for a range of relevant sizes and demonstrate high repeatability for isolated yields at each size selection.

Automation Friendly and Scalable Protocol

Tecan Fluent

Hamilton Microlab STAR

Thermo Kingfisher Flex

The Select-a-Size DNA Clean & Concentrator MagBead Kit is compatible with automated platforms. For automation scripts and related technical support, email tech@zymoresearch.com. In the subject line, please include “Automation Scripts” and include the instrument used and the product catalog number.

Select-a-Size DNA Clean & Concentrator MagBead Kit

Choose from one of the pre-determined cutoffs or fine tune the protocol of these size selection DNA kits for even more specific selections between these points.

Size selections can be performed in as little as 10 minutes to yield high-quality DNA which is suitable for highly sensitive applications including Next Generation Sequencing, and any other sensitive downstream applications.

The protocol of these size selection DNA kits can be performed manually or using an automated platform for high throughput processing.

Frequently Asked Questions

Will this kit work to clean up genomic DNA?

You can use it for genomic clean up, but we recommend the DNA Clean & Concentrator Kit (Catalog No. D4080) instead if it is just genomic DNA. If it is genomic DNA plus smaller DNA fragments, we recommend the Select-a-Size MagBeads.

Will this kit work to remove genomic DNA and recover smaller DNA fragments?

Yes, the Select-a-Size DNA Clean & Concentrator MagBead Kit will work to remove genomic DNA.

Is this a comparable product to AMPure®/SPRI beads?

Yes, the Select-a-Size DNA Clean & Concentrator MagBead Kit is a comparable product.

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