Increased Viral RNA Yields for the Earliest Detection of COVID-19 Outbreaks

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Safe, Affordable SARS-CoV-2 Surveillance

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8x More SARS-CoV-2 RNA

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Zymo Environ Water RNA Kit

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Get 8x More SARS-CoV-2 from Wastewater for the Earliest Detection of COVID-19

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The Zymo Environ Water RNA Kit includes Water Concentrating Buffer (WCB). 5ml of SARS-CoV-2 positive wastewater was processed with and without WCB (n=2). RT-qPCR showed the addition of WCB shifted average Ct values earlier by ~3 (an 8-fold increase in viral recovery).

The Most Effective Wastewater Workflow Ever

  • Enhanced viral enrichment; get 8x more viral RNA
  • Pathogen inactivation for safe handling
  • PCR inhibitors removed in one spin
  • ≥ 6 µl elution improves limits of detection
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PCR Inhibitor-Free RNA

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Extracted Wastewater RNA is free of PCR Inhibitors. 50K HeLa cells were spiked into DNA/RNA Shield (positive extraction control) and into wastewater. RNA was extracted (n=2) and RT-qPCR (Quasar 670) analysis showed the ΔCt is <0.5, indicating the extraction system effectively removes inhibitors in wastewater.

Download the Success Story

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Learn everything about how Los Angeles County Sanitation District successfully tracks COVID-19 surges in wastewater.

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Water Concentrating Buffer Inactivates COVID-19

The Water Concentrating Buffer included in the Zymo Environ Water RNA Kit inactivates SARS-CoV-2 for safe sample processing, while preserving the integrity of viral RNA.

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Viral inactivation demonstrated by plaque formation assay. Vero-E6 cells were infected with serial dilutions of SARS-CoV-2, strain Muc-IMB-1. Virus was applied without pre-treatment, or incubated for 48 hours with agarose overlay before fixation with formaldehyde. Plaques were visualized with crystal violet staining.

FAQ

Yes, the purification steps can be performed on a vacuum manifold. It is recommended that the wash steps and elution steps be performed with a microcentrifuge.
Yes. Filters can be cut or left intact and inserted into the ZR BashingBead™ Lysis Tube. Add 750 µl DNA/RNA Shield and proceed with step 3 of Sample Homogenization.
Wastewater contains solids including cellulose. These solids can bind up viral particles and RNA. Mechanical disruption of solids increases RNA yields dramatically.