Increased Viral RNA Yields for the Earliest Detection of COVID-19 Outbreaks

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8x More SARS-CoV-2 RNA

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Zymo Environ Water RNA Kit

Get 8x More SARS-CoV-2 from Wastewater for the Earliest Detection of COVID-19

The Zymo Environ Water RNA Kit includes Water Concentrating Buffer (WCB). 5ml of SARS-CoV-2 positive wastewater was processed with and without WCB (n=2). RT-qPCR showed the addition of WCB shifted average Ct values earlier by ~3 (an 8-fold increase in viral recovery).
The Most Effective Wastewater Workflow Ever
- Enhanced viral enrichment; get 8x more viral RNA
- Pathogen inactivation for safe handling
- PCR inhibitors removed in one spin
- ≥ 6 µl elution improves limits of detection

PCR Inhibitor-Free RNA

Extracted Wastewater RNA is free of PCR Inhibitors. 50K HeLa cells were spiked into DNA/RNA Shield (positive extraction control) and into wastewater. RNA was extracted (n=2) and RT-qPCR (Quasar 670) analysis showed the ΔCt is <0.5, indicating the extraction system effectively removes inhibitors in wastewater.
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Learn everything about how Los Angeles County Sanitation District successfully tracks COVID-19 surges in wastewater.
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Water Concentrating Buffer Inactivates COVID-19
The Water Concentrating Buffer included in the Zymo Environ Water RNA Kit inactivates SARS-CoV-2 for safe sample processing, while preserving the integrity of viral RNA.

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Beyond SARS-CoV-2 Surveillance
Identify Variants and Vaccine Resistance with the Zymo Environ COVID-19 Wastewater Testing Service

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