Zymo Environ Water RNA Kit
Zymo Environ Water RNA Kit
- Enrich total RNA from large volumes (≤ 5 ml) and transform into small sample pellets for easy processing.
- Inactivates infectious agents and pathogens (viruses, bacteria, fungi, and parasites) for safe handling using DNA/RNA Shield.
- Remove PCR inhibitors in one spin and elute concentrated RNA in ≥6 µl. Ready for any downstream application including RT-qPCR, RTddPCR, NGS.
The Zymo Environ Water RNA Kit simplifies COVID-19 wastewater surveillance by increasing viral enrichment, inactivating pathogens, and improving limits of detection. The use of Water Concentrating Buffer (WCB) promotes the pelleting of viral capsids and protein-associated RNA, increasing SARS-CoV-2 RNA yields by more than 8-fold. DNA/RNA Shield allows for safe handling as it has been validated for inactivation of pathogens including SARS-CoV-2. Finally, the workflow yields highly concentrated, PCR inhibitor-free RNA ready for downstream applications including RT-qPCR, RT-ddPCR, and NGS. Learn more.
|Equipment Needed||Microcentrifuge, vortex, large 50 mL conical tube centrifuge (optional), cell disrupter/pulverizer (optional)|
|Format||Bead beating, spin column.|
|RNA Purity||High quality RNA (A260/A280 > 1.8, A260/A230 > 1.8) suitable for all downstream RNA-based manipulations.|
|RNA Storage||RNA is eluted with RNase-free water and can be stored at ≤-70°C. The addition of RNase inhibitors is highly recommended for prolonged storage.|
|Sample Inactivation||DNA/RNA Shield™ lyses cells, inactivates nucleases and infectious agents, and is ideal for safe sample storage and transport at ambient temperatures.|
|Sample Input||Water, wastewater, sludge, sewage, biofilms, etc.|
|Sample Size||Up to 5 mL|
|Yield||Up to 10 µg RNA can eluted into ≥ 6 µL RNase-free water allowing for a highly concentrated sample.|
“Reliable results and very easy to use. Have been working on wastewater SARS-CoV-2 surveillance for over 9 months now. Tried several methods and kits for concentration of virus particles in wastewater. This kit from Zymo was one of the top performers. What makes it unique is the water concentrating buffer. Will strongly recommend this product."
- Shireen K, University of Virginia, SARS-CoV-2 Surveillance
|R1100-50||DNA/RNA Shield||50 ml||$68.00|
|W1001-6||DNase/RNase-Free Water||6 ml||$16.50|
|D6035-1-30||Prep Solution||30 ml||$19.80|
|R1003-3-24||RNA Wash Buffer||24 ml||$64.90|
|R1013-2-50||RNA Binding Buffer||50 ml||$82.50|
|R1060-2-25||RNA Prep Buffer||25 ml||$44.00|
|C1004-50||Zymo-Spin IC Columns||50 Pack||$58.30|
|C1006-50-G||Zymo-Spin IIICG Columns||50 Pack||$60.50|
|C1058-50||Zymo-Spin III-HRC Filters||50 Pack||$118.80|
|S6012-50||ZR BashingBead Lysis Tubes (0.1 & 0.5 mm)||50 Tubes||$111.10|
|R2042-1-8||Water Concentrating Buffer||8 mL||$7.70|