QuestTaq PreMix

E2050 / E2051


QuestTaq PreMix

E2050 / E2051

Cat # Name Size Price Quantity
E2050 QuestTaq PreMix 50 Rxns $48.00
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E2051 QuestTaq PreMix 200 Rxns. $152.00
+ -

Documents


Data Sheet: PDF   |   Protocol: PDF   |   SDS (MSDS): PDF   |  
Non-biased amplification of 5-mC, 5-hmC, g-5-hmC DNA

Highlights


  • Ideal for robust, non-biased amplification of 5mC, 5hmC, and g5hmC-modified DNA.
  • Premixed reagents for convenient reaction set-up.
  • Compatible with a range of fluorescent dyes for use in real-time PCR.

Description


QuestTaq PreMix is supplied as a convenient 2X concentrated master mix for robust PCR with little to no byproduct formation. It has been optimized for non-biased amplification of cystosine, 5-methylcytosine (5mC), 5-hydroxymethylcytosine (5hmC), and glucosyl-5-hydroxymethylcytosine (g5hmC) containing DNA. It features a unique DNA polymerase and optimized buffer system for amplification of DNA having modified/unmodified cytosines. QuestTaq DNA Polymerase has 3′-terminal transferase activity. The addition of “A” overhangs to amplified DNA makes it ideal for use in TA-cloning.

Technical Specifications


Concentration Reaction conditions at 1X (20 µl total volume) will contain 2 units of QuestTaq DNA polymerase
Source Recombinant Enzyme
Specific Activity 5’ – 3’ polymerization
Storage QuestTaq PreMix should be stored between -20 C to -80 C for maximum performance. Performance is guaranteed for 1 year from the time of receipt.
Unit Definition One unit (U) is defined as the amount of enzyme required for the incorporation of 10 nmol dNTPs into an acid-insoluble form in 30 minutes at 72C.

Kit Components