QuestTaq qPCR PreMix
E2052 / E2053
- Ideal for robust, non-biased amplification of 5mC, 5hmC, and g5hmC-modified DNA
- Provided as a convenient 2X PreMix: just add water, primers, and template DNA.
- Includes a strong fluorescent dye (SYTO 9) for real-time and quantitative PCR applications
QuestTaq qPCR PreMix contains all reagents necessary to perform unbiased quantitative PCR with DNA having 5-methylcytosine (5mC), 5-hydroxymethylcytosine (5hmC), and glucosyl-5-hydroxymethylcytosine (g5hmC) bases. It features a unique DNA polymerase and optimized buffer system for amplification of DNA having modified/unmodified cytosines. It also includes an intense dsDNA-specific fluorescent dye (SYTO 9) for sensitive/precise DNA detection. QuestTaq qPCR PreMix is ideal for locus-specific 5hmC detection when used in conjunction with the Quest 5-hmC Detection Kit (D5410 & D5411) and the Quest 5-hmC Detection Kit-Lite (D5415 & D5416).
|Concentration||Reaction conditions at 1X (20 µl total volume) will contain 2 units of QuestTaq DNA polymerase.|
|Specific Activity||5’ – 3’ polymerization|
|Storage||QuestTaq qPCR PreMix should be stored between -20°C to -80°C for maximum performance. Performance is guaranteed for 1 year from the time of receipt.|
|Unit Definition||One unit (U) is defined as the amount of enzyme required for the incorporation of 10 nmol dNTPs into an acid-insoluble form in 30 minutes at 72°C.|