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Quick-RNA FFPE Miniprep

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Quick-RNA FFPE Miniprep

Cat # Name Size Price Quantity
R1008 Quick-RNA FFPE Miniprep 50 preps $344.20
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Highlights

  • Easy Processing: Included Deparaffinization Solution for simple paraffin removal from FFPE samples. No xylene necessary.
  • Improved Recovery: Optimized Proteinase K digestion and heat ensures maximum recovery of RNA from FFPE tissue samples.
  • High Quality: Non-crosslinked, DNA-free RNA are ready for all downstream applications including PCR, Next-Gen Sequencing, etc.
Description & Documents Specifications Components Related Products

Documents


Protocol: 
Datasheet: 
SDS (MSDS): 

Product Description


The Quick-RNA FFPE Kit provides a simple and reliable method for RNA isolation from formalin-fixed, paraffin embedded (FFPE) tissue samples. The unique chemistries of this FFPE tissue RNA extraction kit have been optimized for maximum recovery of both large and small RNA species. Simply deparaffinize FFPE tissues using Deparaffinization Solution, digest using Proteinase K, heat to reverse chemical crosslinks, and then purify using Zymo-Spin column technology. The FFPE RNA extraction result is high-quality total RNA (including small RNAs 17-200 nt) that is DNA-free and is ready for RT-PCR, hybridization, sequencing, etc.

Documents

Protocol: 
Datasheet: 
SDS (MSDS): 

Technical Specifications

Equipment Microcentrifuge, vortex, heat block/bath
Purity RNA is ready for Next-Gen sequencing, RT-PCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.
Sample Source FFPE Samples: Up to 25 mg tissue from paraffin block or up to four (4) tissue sections (≤20 µm thick) with a total surface area ~20 mm2
Size Range Total RNA ≥ 17 nt
Yield 50 µg RNA (binding capacity), ≥25 µl (elution volume)

Resources


Cat # Name Size Price
W1001-10 DNase/RNase-Free Water 10 ml $22.10
D3067-1-20 Deparaffinization Solution 20 ml $25.60
C1001-50 Collection Tubes 50 Pack $17.50
R1060-2-25 RNA Prep Buffer 25 ml $46.70
R1060-1-50 RNA Lysis Buffer 50 ml $92.20
R1060-1-100 RNA Lysis Buffer 100 ml $176.20
R1003-3-24 RNA Wash Buffer 24 ml $68.80
C1078-50 Zymo-Spin IICR Columns 50 Pack $64.20
D3050-1-5 2X Digestion Buffer - 5 mL 5 mL $11.60
D3050-1-20 2X Digestion Buffer - 20 mL 20 mL $25.60
D3050-1-80 2X Digestion Buffer - 80 mL 80 mL $60.70
E1010-1-4 DNA Digestion Buffer 4 mL $17.50

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