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Direct-zol DNA/RNA Miniprep
R2080T / R2080 / R2081
Direct-zol DNA/RNA Miniprep
- One Input, One Column: Purify RNA and DNA into separate fractions with just one spin-column, directly from a sample in TRIzol, TRI Reagent® or a similar acid-guanidinium-phenol based reagent.
- Easy Handling: Bypass chloroform, phase separation and precipitation steps.
- High-Quality: DNA, RNA (including small/miRNAs) is ready for Next-Gen Sequencing, RT/PCR, hybridization, etc.
The Direct-zol™ DNA/RNA kits provide an innovative method for the purification of DNA and total RNA from a variety of samples freshly lysed in TRIzol or similar, including animal cells, tissue, bacteria, yeast, plant, biological liquids and etc.
Upon lysis of the sample with TRIzol or similar, RNA and DNA is bound directly to the Zymo-Spin™ Column. Then simply spin, wash, and elute high-quality RNA and DNA into separate fractions. No phase separation, precipitation, or post-purification steps are necessary. The eluted nucleic acids are suitable for all subsequent molecular manipulations and analyses including Next-Gen sequencing, RT/qPCR, hybridization, etc.
|Binding Capacity Specification||25 µg DNA and 50 µg RNA (yield will vary with type and treatment of sample)|
|Compatibility||Aqueous (RNA) phase from TRIzol, TRI Reagent® or similar can be processed. Also, compatible with samples in DNA/RNA Shield™ (see Appendix, page 5)|
|Elution Volume||≥ 25 µl|
|Purity||DNA and RNA ready for all subsequent downstream analyses (Next-Gen sequencing, RT/qPCR)|
|Sample Inactivation||TRI Reagent® (provided with R2081, R2083 only) inactivates viruses and other infectious agents.|
|Sample Type Specifications||Cells (animal, bacterial, yeast), tissue (animal, plant) or biological liquids (blood, plasma, serum, CSF, buffy coat, etc.), freshly lysed in TRIzol, TRI Reagent® or similar.|
|Size Range||up to and above 40 kb (DNA), ≥ 17 nt (RNA)|
|C1005-50||Zymo-Spin III Columns||50 Pack||$59.40|
|C1078-50||Zymo-Spin IICR Columns||50 Pack||$60.50|
|R2080-1-24||Direct-zol DNA Wash 1 (concentrate)||24 ml||$31.90|
|R2080-2-40||Direct-zol DNA Wash 2||40 ml||$31.90|
|R2080-3-4||Direct-zol DNA Elution Buffer||50 preps||$14.30|
|R2050-1-50||TRI Reagent||50 ml||$79.20|
|R1060-2-25||RNA Prep Buffer||25 ml||$44.00|
|E1010||DNase I Set||250 U||$61.60|
|R1003-3-12||RNA Wash Buffer||12 ml||$33.00|
|W1001-1||DNase/RNase-Free Water||1 ml||$11.00|
|W1001-4||DNase/RNase-Free Water||4 ml||$13.20|