ZymoBIOMICS Microbial Community Standard II (Log Distribution)

D6310


ZymoBIOMICS Microbial Community Standard II (Log Distribution)

D6310

Cat # Name Size Price Quantity
D6310 ZymoBIOMICS Microbial Community Standard II (Log Distribution) 10 Preps $300.00
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Documents


Microbial and DNA standard with a log distribution used to assess the performance of microbiomics workflows.

Highlights


  • Log abundance distribution: assess detection limit over a broad range (102 to 108 cells)
  • Accurate composition: assess the accuracy of microbiome measurements
  • Microbiomics QC: ideal for quality control of microbiome measurements

Description


ZymoBIOMICS Microbial Community Standard II (Log Distribution) is a mock microbial community consisting of eight bacterial and two fungal strains. This microbial standard can be used to assess the performance of microbiomics workflows and can also be used as a positive control for routine sequencing. Cells of the ten microbes were mixed in a log-distributed abundance, which allows the user to easily assess the detection limit of their microbiomics workflow. 75 µl of the standard contains about ~100 cells of Staphylococcus aureus, the organism of lowest abundance. If needed, the standard can be spiked into a sample matrix (e.g. soil and blood) to mimic real samples of interest. Theoretical Composition Based on Genomic DNA: Listeria monocytogenes - 89.1%, Pseudomonas aeruginosa - 8.9%, Bacillus subtilis - 0.89%, Saccharomyces cerevisiae - 0.89%, Escherichia coli - 0.089%, Salmonella enterica - 0.089%, Lactobacillus fermentum - 0.0089%, Enterococcus faecalis - 0.00089%, Cryptococcus neoformans - 0.00089%, and Staphylococcus aureus - 0.000089%.

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Technical Specifications


Applicable For NGS, microbiomics, metagenomics.
Processing Volume 75 µl per preparation.
Purity Contains < 0.01% foreign microbial DNA.
Sample Source Eight bacteria (3 Gram-negative and 5 Gram-positive) and 2 yeasts.
Yield Approximately 220 ng DNA per preparation.

Citations