ZymoBIOMICS HMW DNA Standard
- Long-Read Sequencing Ready: Ideal to benchmark 3rd generation sequencing and metagenomic measurements (e.g. Oxford Nanopore & PacBio).
- High Molecular Weight: DNA standard is >50 kb in size.
- Accurate Composition: Composition is cross-validated with multiple types of measurements.
ZymoBIOMICS HMW DNA Standard is a mixture of high molecular weight genomic DNA isolated from pure cultures of seven bacterial and one fungal strains. It has an accurately defined composition, negligible impurities (<0.01%) and contains genomes of a wide range of GC content (15%-85%). This can be used to assess and benchmark the performance of long read/3rd generation sequencing for microbiomics and metagenomics analysis and can also be used as a routine quality control. Theoretical Composition Based on Genomic DNA: Pseudomonas aeruginosa - 14%, Escherichia coli - 14%, Salmonella enterica - 14%, Enterococcus faecalis - 14%, Staphylococcus aureus - 14%, Listeria monocytogenes - 14%, Bacillus subtilis - 14%, Saccharomyces cerevisiae - 2%.
|Impurity Level||<0.01% foreign microbial DNA|
|Source||Seven bacteria (3 gram-negative and 4 gram-positive) and 1 yeast.|
|Storage Solution||10 mM Tris-HCl and 0.1 mM EDTA, pH 8.0|