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    Spike-in Controls | High Microbial Load

    ZymoBIOMICS Spike-in Controls provide in situ controls for assessing recovery and absolute abundance in mixed-microbial samples.

    Format

    ZymoBIOMICS Spike-in Control I (High Microbial Load)

    Highlights

    • Absolute Quantification: enables absolute cell number measurements using Next-Generation Sequencing
    • in situ Quality Control: ensures each sample is quantified accurately
    • Unique composition: comprised of two microbes alien to the human microbiome
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    Format

    ZymoBIOMICS Spike-in Control I (High Microbial Load)

    Highlights

    • Absolute Quantification: enables absolute cell number measurements using Next-Generation Sequencing
    • in situ Quality Control: ensures each sample is quantified accurately
    • Unique composition: comprised of two microbes alien to the human microbiome
    Zymo Research's 100% Satisfaction Guarantee

    Original Manufacturer

    Innovated in California, Made in the USA

    Satisfaction 100% guaranteed, read Our Promise

    Cat # Name Size Price Quantity
    D6320 ZymoBIOMICS Spike-in Control I (High Microbial Load) 25 Preps $114.30
    - +
    D6320-10 ZymoBIOMICS Spike-in Control I (High Microbial Load) 250 Preps $582.40
    - +

    Description

    ZymoBIOMICS™ Spike-in Control I (High Microbial Load) consists of equal cell numbers of two bacteria strains, Imtechella halotolerans and Allobacillus halotolerans. When spiked into an unknown sample, this product will serve as an in situ positive control for DNA-sequencing-based (e.g. NGS-based) microbiome measurements. These two bacteria, Imtechella halotolerans (Gram-negative) and Allobacillus halotolerans (Gram-positive) represent different cell recalcitrance and can expose potential bias during DNA extraction. Moreover, with accurately quantified cell numbers, this standard enables absolute cell number quantification in microbiome measurements.

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    Performance

    Technical Specifications
    Biosafety This product is not biohazardous as microbes have been fully inactivated.
    Impurity Level < 0.01% foreign microbial DNA
    Reference Genomes Complete reference genomes and 16S rRNA genes
    Sample Storage -80°C
    Storage Solution DNA/RNA Shield

    Resources

    Documents

    FAQ

    Studies where strict bioburden is required, such as food or water studies, it would be useful to use the Spike-in Control.

    High biomass samples (feces, cell culture, etc.) should be used with Spike-In I.

    Low Biomass samples (swabs, water filters, etc.) should be used with Spike-In II.

    All bacterial sample types are compatible with the spike-in control.

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