Microbiome Standards

Validate your microbiome workflow

Cartoon cell
  • Assess Bias in Microbiome Measurements
  • Defined Composition
  • Test the Detection Limit of Workflows

Evaluate Microbiome Workflows

Assess and Eliminate Bias

ZymoBIOMICS Microbial Composition graph

DNA from the ZymoBIOMICS Microbial Standard was extracted using four different methods: ZymoBIOMICS DNA Miniprep Kit (middle-left), HMP fecal DNA extraction protocol (middle), Supplier M (middle-right), and Supplier Q (right). Duplicates were performed. The extracted DNA was sequenced by targeted 16S rRNA gene sequencing. The theoretical/defined composition of the standard is shown on the left.


Accurately Defined Composition

Ahead of the Competition

ZymoBIOMICS Microbial Standards Supplier A
Abundance Variation < 15% < 1,000%
Foreign Contaminants < 0.01% < 10%
Include both Bacteria and Fungi
Strict Manufacturing QC

Defined. Quantified. Accurate.

Shotgun Sequencing ZymoBiomics Bar Graph

Microbial composition of ZymoBIOMICS standards determined by Shotgun Sequencing.
For the microbial standard (D6300), DNA was extracted using the ZymoBIOMICS DNA Miniprep kit. The library preparation was performed using Kapa HyperPlus kit. Sequencing was performed with Illumina HiSeq. Theoretical abundance (left) is given for comparison.


Low Impurities

contamination graph

Foreign microbial DNA contamination assessed with deep shotgun sequencing.
In this test, 178 Million Illumina paired-end reads were generated. P. acnes (bottom) was the only foreign organism detected.


Sensitive and Accurate

Test the Detection Limit of Workflows

Log-Distributed Abundance

Detection Limits DNA abundance

ZymoBIOMICS Microbial Community DNA Standard II (D6310) can assess both the detection limit and accuracy of your workflow over a broad-range (102-108 cells). This figure plots the composition of the standard as measured by NGS shotgun sequencing against the defined composition of the standard.

Test 16S Sequencing Workflows

zymobiomics community standard graph

ZymoBIOMICS Microbial Community DNA Standard II (D6311) was processed through 16S rRNA gene targeted sequencing and the sequencing data was analyzed using two bioinformatics pipelines, QIIME (red) and Dada2 (green). The theoretical abundance (blue) is displayed to facilitate comparison. The performance can be assessed regarding accuracy, false positive rates, and detection limit.


Rigorously Test Workflows

Microbial and DNA formats

cartoon microbial cells

Microbial
(assess total workflow biases)

Cartoon yellow dna strand

Extracted DNA
(Assess Library preparation biases)


Ensure Accurate Discoveries

Product Catalog # Composition Format Manual
Microbial Community Standard D6300 Even Distribution Microbial Instruction manuel image
Microbial Community DNA Standard D6305/6306 Even Distribution Isolated DNA Instruction manuel image
Microbial Community Standard II D6310 Log Distribution Microbial Instruction manuel image
Microbial Community DNA Standard II D6311 Log Distribution Isolated DNA Instruction manuel image
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