ZymoBIOMICS Microbial Community DNA Standard

D6305 / D6306


ZymoBIOMICS Microbial Community DNA Standard

D6305 / D6306

Cat # Name Size Price Quantity
D6305 ZymoBIOMICS Microbial Community DNA Standard 200ng $104.00
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D6306 ZymoBIOMICS Microbial Community DNA Standard 2000ng $208.00
+ -

Documents


Datasheet: PDF   |   Protocol: PDF   |   SDS (MSDS): PDF   |  
ZymoBIOMICS® Microbial Community Isolated DNA Standard

Highlights


  • Accurate composition: composition cross-validated with multiple types of measurements.
  • Negligible impurity: guaranteed to contain < 0.01% foreign microbial DNA.
  • Wide range of GC content: 15%-85%, for assessing bias caused by GC content variation.

Description


One of the major challenges in the emerging field of microbiomics is the bias and errors introduced in the complex workflows. Besides nucleic acid purification, bias also arises from sequencing library preparation and subsequent processes. The ZymoBIOMICS Microbial Community DNA Standard is designed to assess bias, errors and other artifacts after the step of nucleic acid purification. This DNA standard is created by pooling DNA extracted from pure cultures. It has an accurately defined composition, negligible impurities (0.01%), and contains genomes of a wide range of GC content (15% - 85%). This DNA standard is designed to have the same microbial composition with the cellular version, the ZymoBIOMICS Microbial Community Standard, so that they can be more powerful when working in tandem. Theoretical Composition Based on Genomic DNA: Listeria monocytogenes - 12%, Pseudomonas aeruginosa - 12%, Bacillus subtilis - 12%, Escherichia coli - 12%, Salmonella enterica - 12%, Lactobacillus fermentum - 12%, Enterococcus faecalis - 12%, Staphylococcus aureus - 12%, Saccharomyces cerevisiae - 2%, and Cryptococcus neoformans - 2%.

Technical Specifications


Purity < 0.01% foreign microbial DNA
Sample Source A mixture of genomic DNA of ten microbial strains.
Sample Storage -20 °C