Quick-DNA 96 Plus Kit
D4070 / D4071
- Extract high-quality DNA easily and reliably from any biological fluids, cultured/monolayer cells, or solid tissues.
- Zymo-Spin Technology ensures DNA is ready for all sensitive downstream applications such as qPCR, DNA-sequencing, arrays, and methylation analysis.
The Quick-DNA 96 Plus Kits are the easiest DNA isolation kits for high yield total DNA extraction (e.g., genomic, mitochondrial, viral) from cell culture, solid tissue, saliva, and any biological fluid sample. Innovative reagents and Zymo-Spin Technology allow for ultra-pure and concentrated genomic DNA (> 50 kb) to be eluted in as little as 10 µl. Zymo-Spin Columns ensure no buffer retention. Purified DNA is RNA-free bypassing the need for RNase A treatment and ensuring accurate quantification for applications like library preparations. Isolated DNA is suitable for immediate use in sensitive downstream applications including qPCR, DNA-seq, arrays, and methylation analysis.
|Elution Volume||≤ 15 µl|
|Equipment||Water bath or heat block (55°C), microcentrifuge, and vortex.|
|Purity||High quality DNA is ready for all sensitive downstream applications such as PCR, endonuclease digestion, Southern blotting, genotyping, Next-Gen sequencing, bisulfite conversion, etc. (A260/A230≥ 2.0).|
|Size Range||Capable of recovering genomic and mitochondrial DNA sized fragments > 50 kb. If present, parasitic, microbial, and viral DNA will also be recovered.|
|Workflow||Utilize a Proteinase K Digestion and Zymo-Spin Column for effective recovery of DNA.|
|Yield||The DNA binding capacity of the well is 5 µg. Typically, mammalian tissues yield: 1-3 µg DNA per mg skeletal, heart, lung, and brain tissues and 3-5 µg DNA per mg liver and kidney. Human whole blood will yield 3-7 µg DNA per 100 µl blood sampled.|