Quick-RNA Midiprep Kit
Quick-RNA Midiprep Kit
- Broad Range: Extract total RNA (including small/micro RNAs) from any sample.
- DNA-Free: Genomic DNA removal column and DNase I included.
- NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.
The Quick-RNA Midiprep Kit is one of the most innovative RNA isolation kits available, designed for the easy, reliable, and rapid isolation of up to 1 mg total RNA from cultured cells or tissue samples. The procedure combines a unique, single-step RNA extraction/binding buffer with Clean-Spin™ column technology to yield high quality RNA in about 10 minutes. The Quick-RNA™ Midiprep Kit allows for the efficient recovery of total RNA from 103 to 108 cells or tissue.
The method is easy: simply add the provided ZR RNA Buffer to extract total RNA
from the cells of interest then purify the RNA using the provided Zymo-Spin™ V-E Columns. The result is highly-concentrated, purified RNA that is suitable for subsequent RNA-based methods including RT-PCR, hybridization, etc.
|Equipment||Microcentrifuge, vortex, vacuum manifold|
|Purity||RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.|
|Sample Source||Cells or tissue samples, yeast, plant or bacteria. Compatible with DNA/RNA Shield™ and RNAlater™.|
|Size Range||Total RNA ≥ 17 nt|
|Yield||Midiprep: 1 mg RNA (binding capacity), ≥ 200 µl (elution volume)|
Q1: What is the difference between the Quick-RNA Miniprep and the Quick-RNA Miniprep Plus?
Use the Quick-RNA Miniprep for cells and soft tissues. The “Plus” kit accommodates all sample types (cells, tissue, blood) and comes with DNA/RNA Shield (sample collection, transport, storage at ambient temperature).
Q2: Is DNase I available for individual purchase?
Yes, the catalog number for the DNase I set (DNase and DNA Digestion Buffer) that we offer is E1010.
Q3: Is the DNase-I treatment necessary?
If the downstream application requires DNA-free RNA, we recommend performing the DNase I treatment.
Q4: How to store DNase-I following resuspension?
Lyophilized DNase I is stable at room temperature. Once resuspended, store frozen aliquots. Minimize freeze thaw cycles as much as possible. Freeze thaw will lower DNase activity.
Q5: I ran out of RNA Wash Buffer. Can I use something else?
Yes. Use 80% ethanol as a substitute. RNA Wash Buffer is also sold separately.
Q6: Will the kit isolate small RNAs?
Yes, this kit will isolate small/micro RNA’s ≥ 17 nucleotides.
Q7: Can samples be stored in RNA Lysis Buffer prior to processing?
Yes, samples in RNA Lysis Buffer are stable overnight at room temperature and can be stored frozen (-80C). Be sure to lyse and homogenize the sample well prior to freezing. Bring the sample to room temperature prior to RNA Purification.
Q8: What is the difference between the Direct-zol RNA and Quick-RNA kits?
Direct-zol is for samples stored/collected into TRIzol/similar reagents. Quick-RNA is for all other samples.
Q9: Is it possible to extract proteins with the Quick-RNA kit?
Yes, proteins can be acetone precipitated from the column flowthrough. Please request supplementary protocol from Zymo Research Technical Support.
"RNA isolation from tissue culture cells used to be my most hated protocol - labor intensive, tedious, and the horrible smell of the BME used in the protocol. This kit is much faster and easier and no horrible smells! Both the RNA yield and the real time results are just as good as our previous kit."
- Lisa G. (University of Virginia)
“Amazing results, the only RNA extraction kit I ever buy now. In my opinion, there is no product on the market that is as good a value and as effective as this kit for plant tissue RNA extractions.”
- Erin B. (Occidental College)
"Good price and easy to use, plus good quality. It is compatible to QIAGEN products RNeasy kit, but with reasonable price and equal quality. Especially the DNase is inexpensive and is included in the kit. No matter animal RNA, or plant RNA, or bacterial RNA, all could use with this one kit."
- Xiaolu J. (Florida Atlantic University)
|W1001-10||DNase/RNase-Free Water||10 ml||$19.00|
|C1001-50||Collection Tubes||50 Pack||$15.00|
|R1003-3-6||RNA Wash Buffer||6 ml||$18.00|
|R1020-1-100||ZR RNA Buffer||100 ml||$140.00|
|R1020-2-12||RNA Pre-Wash Buffer||12 ml||$21.00|