Direct-zol RNA Microprep Kits
R2062 / R2061 / R2063 / R2060
- Easy Handling: Bypass chloroform, phase separation and precipitation steps.
- NGS-Ready: Ultra-pure RNA without phenol carryover. No DNA contamination (DNase I included).
- Non-Biased: Complete RNA recovery without miRNA loss.
|Compatibility||TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®.|
|Sample Inactivation||TRI Reagent® (provided with R2061, R2063) inhibits RNase activity and inactivates viruses and other infectious agents.|
|Sample Source||Any sample stored and preserved in TRI Reagent®, TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)).|
|Size Range||Total RNA ≥ 17 nt|
|Yield||10 µg RNA (binding capacity), ≥6 µl (elution volume)|
Q1: Is Direct-zol suitable for very small numbers of cells?
Q2: Is DNase I available for individual purchase?
Q3: How to store DNase-I following resuspension?
Q4: Is the DNase-I treatment necessary?
Q5: Is the kit compatible with samples stored in DNA/RNA Shield?
Q6: Is it possible to extract proteins with the Direct-zol RNA kits?
Q7: I ran out of RNA Wash Buffer. Can I use something else?
Q8: Can samples be stored in TRIzol/TRI Reagent prior to processing?
Q9: Is it possible to isolate DNA with the Direct-zol RNA kits?
Q10: Is the RNA suitable for Next-Gen sequencing or other sensitive downstream applications?
Q11: Which phenol-based reagents are compatible with Direct-zol?
Q12: What is the difference between the Direct-zol RNA and Quick-RNA kits?
Q13: What is the difference between the Direct-zol RNA MiniPrep and the Direct-zol RNA MiniPrep Plus?
Researchers used the Direct-zol RNA MiniPrep for heterologous E. coli expression of the FnCpf1 locus by means of RNA-seq and discovered a new endonuclease, Cpf1, which further improves upon the widely used CRISPR-Cas9 genome editing system. Cpf1 targets distinct PAM sequences, requires no tracrRNA, and cleaves DNA with staggered overhangs.Zetsche B, et. al. (2015). Cpf1 is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell. 163(3): 759-71.
The Direct-zol RNA Miniprep kit was used to isolate pure total RNA from Rat cells, and the high quality RNA was used to create a cDNA library for RT-qPCR analysis of several genes related to hematopoietic Stem Cell (HSC) Development. The consistent RNA extraction allowed the scientists to determine that HSC cells do not significantly contribute to kidney repair following acute kidney injuries.Burst V. et al. (2012) Survival and distribution of injected haematopoietic stem cells in acute kidney injury. Nephrol Dial Transplant 0: 1–8
High-quality RNA isolated with the Direct-zol™ RNA MiniPrep from fecal and culture samples were shown to be instrumental in the development of a rotavirus early detection system using RT-PCR. The efficient RNA isolation helps in identification of severe gastroenteritis in infants and young children.Yoshiki F, Takashi S, Takagi H, et al. Amplification of all 11 RNA segments of group A rotaviruses based on reverse transcription polymerase chain reaction Microbiol Immunol 2012; 56:
“Before I discovered this kit, I was isolating RNA the old school way with chloroform and it would take half the day to finish the protocol. The Direct-zol RNA Miniprep kit is AWESOME! It took hardly any time, the protocol was so easy, and my RNA quality was SO much better. Honestly, this kit revolutionized my life at the bench.”
- A. Newhart (The Wistar Institute)
“Simple protocol and yielded good quality of RNA. Only one kit working for all type of tissue, cell and especially biological fluids.”
- Mohan K. (University of Illinois, Chicago)
“Previously I used a protocol that took 3 hours, now I can have my RNA in 20 minutes. What is not to like about that? Just one column and two buffers, I love it.”
- Arjan V. (Indiana University)