Direct-zol-96 MagBead RNA

R2104 / R2103 / R2102 / R2100 / R2105 / R2101


Direct-zol-96 MagBead RNA

R2104 / R2103 / R2102 / R2100 / R2105 / R2101

Cat # Name Size Price Quantity
R2104 Direct-zol-96 MagBead RNA 8 x 96 preps $1,085.00
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R2103 Direct-zol-96 MagBead RNA (Product Supplied w/ 200 ml x 2 TRI Reagent) 4 x 96 preps $1,102.00
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R2102 Direct-zol-96 MagBead RNA 4 x 96 preps $684.00
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R2100 Direct-zol-96 MagBead RNA 2 x 96 preps $422.00
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R2105 Direct-zol-96 MagBead RNA (Product Supplied w/ 200 ml x 4 TRI Reagent) 8 x 96 preps $1,915.00
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R2101 Direct-zol-96 MagBead RNA (Product Supplied w/ 200 ml TRI Reagent) 2 x 96 preps $635.00
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Documents


Isolate total RNA and small/miRNA from TRIzol without phase separation

Highlights


  • Easy Handling:Bypass chloroform, phase separation and precipitation steps.
  • NGS-Ready: Ultra-pure RNA without phenol carryover. No DNA contamination (DNase I included).
  • Non-Biased: Complete RNA recovery without miRNA loss.

Description


The Direct-zol™-96 MagBead RNA kit provides a high-throughput (96-well), magnetic bead-based isolation of high-quality RNA directly from samples in TRI Reagent® and similar. The extraction method inactivates viruses and other infectious agents. Total RNA including small/microRNAs (≥17 nt) are effectively isolated from a variety of sample sources (cells, tissues, biological liquids, etc.). The procedure is easy: simply add ethanol and MagBinding Beads to a sample in TRI Reagent®, wash and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including Next-Gen sequencing, RT-PCR, transcription profiling, hybridization, etc.

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Technical Specifications


Compatibility TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®.
Equipment Microcentrifuge, vortex, magstand
Sample Inactivation TRI Reagent® (provided with R2101, R2103, and R2105) inhibits RNase activity and inactivates viruses and other infectious agents.
Sample Source Any sample stored and preserved in TRI Reagent®, TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)).
Size Range Total RNA ≥ 17 nt
Yield 10 µg RNA (binding capacity), ≥ 30 µl (elution volume)

Product FAQ


Q1: Is Direct-zol suitable for very small numbers of cells?

Q2: Is DNase I available for individual purchase?

Q3: How to store DNase-I following resuspension?

Q4: Is the DNase-I treatment necessary?

Q5: Is the kit compatible with samples stored in DNA/RNA Shield?

Q6: Is it possible to extract proteins with the Direct-zol RNA kits?

Q7: Can samples be stored in TRIzol/TRI Reagent prior to processing?

Q8: Is it possible to isolate DNA with the Direct-zol RNA kits?

Q9: Is the RNA suitable for Next-Gen sequencing or other sensitive downstream applications?

Q10: Which phenol-based reagents are compatible with Direct-zol?

Q11: What is the difference between the Direct-zol RNA and Quick-RNA kits?

Q12: What is the difference between the Direct-zol RNA MiniPrep and the Direct-zol RNA MiniPrep Plus?

Q13: I ran out of RNA Wash Buffer. Can I use something else?

Product Video


Citations


Researchers used the Direct-zol RNA MiniPrep for heterologous E. coli expression of the FnCpf1 locus by means of RNA-seq and discovered a new endonuclease, Cpf1, which further improves upon the widely used CRISPR-Cas9 genome editing system. Cpf1 targets distinct PAM sequences, requires no tracrRNA, and cleaves DNA with staggered overhangs.

Zetsche B, et. al. (2015). Cpf1 is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell. 163(3): 759-71.

The Direct-zol RNA Miniprep kit was used to isolate pure total RNA from Rat cells, and the high quality RNA was used to create a cDNA library for RT-qPCR analysis of several genes related to hematopoietic Stem Cell (HSC) Development. The consistent RNA extraction allowed the scientists to determine that HSC cells do not significantly contribute to kidney repair following acute kidney injuries.

Burst V. et al. (2012) Survival and distribution of injected haematopoietic stem cells in acute kidney injury. Nephrol Dial Transplant 0: 1–8

High-quality RNA isolated with the Direct-zol™ RNA MiniPrep from fecal and culture samples were shown to be instrumental in the development of a rotavirus early detection system using RT-PCR. The efficient RNA isolation helps in identification of severe gastroenteritis in infants and young children.

Yoshiki F, Takashi S, Takagi H, et al. Amplification of all 11 RNA segments of group A rotaviruses based on reverse transcription polymerase chain reaction Microbiol Immunol 2012; 56:

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Reviews


“Before I discovered this kit, I was isolating RNA the old school way with chloroform and it would take half the day to finish the protocol. The Direct-zol RNA Miniprep kit is AWESOME! It took hardly any time, the protocol was so easy, and my RNA quality was SO much better. Honestly, this kit revolutionized my life at the bench.”

- A. Newhart (The Wistar Institute)

“Simple protocol and yielded good quality of RNA. Only one kit working for all type of tissue, cell and especially biological fluids.”

- Mohan K. (University of Illinois, Chicago)

“Previously I used a protocol that took 3 hours, now I can have my RNA in 20 minutes. What is not to like about that? Just one column and two buffers, I love it.”

- Arjan V. (Indiana University)

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Kit Components