- Easy Handling: Bypass chloroform, phase separation and precipitation steps.
- NGS-Ready: Ultra-pure RNA without phenol carryover. No DNA contamination (DNase I included).
- Non-Biased: Complete RNA recovery without miRNA loss.
|Compatibility||TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®.|
|Sample Inactivation||TRI Reagent® (provided with R2051, R2053) inhibits RNase activity and inactivates viruses and other infectious agents.|
|Sample Source||Any sample stored and preserved in TRI Reagent®, TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)).|
|Size Range||Total RNA ≥ 17 nt|
|Yield||50 µg RNA (binding capacity), ≥25 µl (elution volume)|
Q1: Is DNase I available for individual purchase?
Q2: How to store DNase-I following resuspension?
Q3: Is the kit compatible with samples stored in DNA/RNA Shield?
Q4: Is Direct-zol suitable for very small numbers of cells?
Q5: Is it possible to extract proteins with the Direct-zol RNA kits?
Q6: Is the DNase-I treatment necessary?
Q7: Can samples be stored in TRIzol/TRI Reagent prior to processing?
Q8: Is it possible to isolate DNA with the Direct-zol RNA kits?
Q9: Is the RNA suitable for Next-Gen sequencing or other sensitive downstream applications?
Q10: Which phenol-based reagents are compatible with Direct-zol?
Q11: What is the difference between the Direct-zol RNA and Quick-RNA kits?
Q12: What is the difference between the Direct-zol RNA MiniPrep and the Direct-zol RNA MiniPrep Plus?
Q13: I ran out of RNA Wash Buffer. Can I use something else?
“No phase separation was needed, but you still had the benefits of a Trizol extraction. No need to precipitate and resuspend samples, which means less sample loss during purification.”
- Adina B. (University of Guelph)
“This kit is amazing, I've got a gel comparing the lack of gDNA as shown in the advertising pamphlet. What can I say, except: I love this product!“
- R.K. CSU
“Direct-zol is the most excellent kit for RNA isolation that I ever used in the past 20 years.”
- H.Z. (Harvard Medical School)