Direct-zol RNA Miniprep Kits

R2050 / R2051 / R2052 / R2053


Direct-zol RNA Miniprep Kits

R2050 / R2051 / R2052 / R2053

Cat # Name Size Price Quantity
R2050 Direct-zol RNA Miniprep 50 preps $180.00
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R2051 Direct-zol RNA Miniprep (Product Supplied w/ 50 ml TRI Reagent) 50 preps $250.00
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R2052 Direct-zol RNA Miniprep 200 preps $555.00
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R2053 Direct-zol RNA Miniprep (Product Supplied w/ 200 ml TRI Reagent) 200 preps $770.00
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Documents


Isolate total RNA and small/miRNA from TRIzol without phase separation

Highlights


  • Easy Handling: Bypass chloroform, phase separation and precipitation steps.
  • NGS-Ready: Ultra-pure RNA without phenol carryover. No DNA contamination (DNase I included).
  • Non-Biased: Complete RNA recovery without miRNA loss.

Description


The Direct-zol™ RNA MiniPrep Kits are RNA purification kits that provide a streamlined method for the purification of up to 50 µg (per prep) of high-quality RNA directly from samples in TRI Reagent® or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.). Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol™ method assures unbiased recovery of small RNAs including miRNA. The procedure is easy. Simply apply a prepared sample in TRI Reagent® directly to the Zymo-Spin™ Column and then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The eluted RNA is high quality and suitable for subsequent molecular manipulation and analysis (including RT-PCR, transcription profiling, hybridization, sequencing etc.). The entire procedure typically takes only 7 minutes.

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Technical Specifications


Compatibility TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®.
Equipment Microcentrifuge, vortex
Sample Inactivation TRI Reagent® (provided with R2051, R2053) inhibits RNase activity and inactivates viruses and other infectious agents.
Sample Source Any sample stored and preserved in TRI Reagent®, TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)).
Size Range Total RNA ≥ 17 nt
Yield 50 µg RNA (binding capacity), ≥25 µl (elution volume)

Product FAQ


Q1: Is DNase I available for individual purchase?

Q2: How to store DNase-I following resuspension?

Q3: Is the kit compatible with samples stored in DNA/RNA Shield?

Q4: Is Direct-zol suitable for very small numbers of cells?

Q5: Is it possible to extract proteins with the Direct-zol RNA kits?

Q6: Is the DNase-I treatment necessary?

Q7: Can samples be stored in TRIzol/TRI Reagent prior to processing?

Q8: Is it possible to isolate DNA with the Direct-zol RNA kits?

Q9: Is the RNA suitable for Next-Gen sequencing or other sensitive downstream applications?

Q10: Which phenol-based reagents are compatible with Direct-zol?

Q11: What is the difference between the Direct-zol RNA and Quick-RNA kits?

Q12: What is the difference between the Direct-zol RNA MiniPrep and the Direct-zol RNA MiniPrep Plus?

Q13: I ran out of RNA Wash Buffer. Can I use something else?

Product Video


Citations


Reviews


“No phase separation was needed, but you still had the benefits of a Trizol extraction. No need to precipitate and resuspend samples, which means less sample loss during purification.”

- Adina B. (University of Guelph)

“This kit is amazing, I've got a gel comparing the lack of gDNA as shown in the advertising pamphlet. What can I say, except: I love this product!“

- R.K. CSU

“Direct-zol is the most excellent kit for RNA isolation that I ever used in the past 20 years.”

- H.Z. (Harvard Medical School)

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Kit Components