Direct-zol-96 RNA Kits

R2054 / R2055 / R2056 / R2057


Direct-zol-96 RNA Kits

Cat # Name Size Price Quantity
R2054 Direct-zol-96 RNA 2 x 96 preps $459.80
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R2055 Direct-zol-96 RNA (Product Supplied w/ 200 ml x 1 TRI Reagent) 2 x 96 preps $690.80
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R2056 Direct-zol-96 RNA 4 x 96 preps $742.50
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R2057 Direct-zol-96 RNA (Product Supplied w/ 200 ml x 2 TRI Reagent) 4 x 96 preps $1,215.50
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Highlights

  • Easy Handling: Bypass chloroform, phase separation and precipitation steps.
  • NGS-Ready: Ultra-pure RNA without phenol carryover. No DNA contamination (DNase I included).
  • Non-Biased: Complete RNA recovery without miRNA loss.
Description

The Direct-zol-96 RNA Kits are RNA purification kits that provide a streamlined method for the purification of up to 10 µg (per well) of high-quality RNA directly from samples in TRI Reagent®. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.) using this product. The extraction method inactivates viruses and other infectious agents. The procedure is easy: simply apply a sample in TRI Reagent® to the Zymo-Spin I-96 Plate, then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including RT-PCR, transcription profiling, hybridization, etc. The entire procedure typically takes about 30 minutes (per 2 plates).

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Compatibility TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®.
Equipment Microplate centrifuge, vortex
Sample Inactivation TRI Reagent® (provided with R2055, R2057) inhibits RNase activity and inactivates viruses and other infectious agents.
Sample Source Any sample stored and preserved in TRI Reagent®, TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)).
Size Range Total RNA ≥ 17 nt
Supplemental Info
Yield 10 µg RNA (binding capacity), ≥10 µl (elution volume)

All kit components are available for purchase separately.

Lyophilized DNase I is stable at room temperature. Once resuspended, store frozen aliquots. Minimize freeze thaw cycles as much as possible. Freeze thaw will lower DNase activity.

If the downstream application requires DNA-free RNA, we recommend performing the DNase I treatment.

Yes, bring samples homogenized and stored in DNA/RNA Shield to room temperature (20-30ºC). Add 3 volume of TRIzol/TRI Reagent and mix well. Proceed with RNA Purification.

Yes, the Direct-zol MicroPrep (#R2060) is designed and capable of purifying RNA down to single cell inputs (picogram amounts). A sensitive quantification method is needed (e.g. Qubit, qPCR, etc.)

Yes, proteins can be acetone precipitated from the column flowthrough. Please see the Protein Purification appendix in the protocol.

Yes, samples in TRIzol/TRI Reagent or similar are stable overnight at room temperature and can be stored frozen (-80C). Be sure to lyse and homogenize the sample well prior to freezing. Bring the sample to room temperature prior to RNA Purification.

Direct-zol DNA/RNA (R2080) kits can isolate DNA from TRIzol.

Yes, the RNA is high quality (A260/A280 >1.8, A260/A230 >1.8) and suitable for any downstream application, including NGS, RT-PCR, hybridization, etc.

The Direct-zol kits are compatible with TRI Reagent, TRIzol, Qiazol, RNAzol, TriPure, TriSure, etc., and any other acid-guanidinium phenol-based reagents.

Direct-zol is for samples stored/collected into TRIzol/similar reagents. Quick-RNA is for all other samples.

Both kits function the same, the only difference is the RNA binding capacity of the column provided with the kit.

Yes, use 80% ethanol as a substitute. RNA Wash Buffer is also sold separately.

Input Average RNA Yield
Cells 10 µg (per 106 cells)
HeLa 15 µg
High Yield Tissue (mouse) ≥ 30 µg (per 10 mg)
Spleen 30-50 µg
Liver 40-60 µg
Low Yield Tissue (mouse) ≤ 30 µg (per 10 mg)
Brain, Heart 5-15 µg
Muscle 5-20 µg
Lung 10-20 µg
Intestine 10-30 µg
Kidney 20-30 µg
Whole Blood (per 1 ml)
Porcine 10-20 µg
Human 2-10 µg

Purity, RIN and/or any type of contamination can result from initial sample preparation (i.e., inefficient lysis of the sample). To improve, increase the volume of the lysis reagent (e.g., TRI Reagent/TRIzol or RNA Lysis Buffer).

  • To ensure complete lysis, increase the volume of the lysis reagent (i.e., increase or titrate the volume of TRI Reagent/TRIzol or RNA Lysis Buffer). Lysate should be clear (not opaque or viscous). Pellet debris by centrifugation (if needed) and process the cleared supernatant.
  • Prior to adding TRI Reagent/TRIzol or RNA Lysis Buffer, perform enzymatic treatment (Proteinase K; #D3001-2-20) and/or mechanical homogenization (bead beating with ZR BashingBead Lysis Tubes; #S6003) in DNA/RNA Shield (#R1100).



“No phase separation was needed, but you still had the benefits of a Trizol extraction. No need to precipitate and resuspend samples, which means less sample loss during purification.”

- Adina B. (University of Guelph)

“This kit is amazing, I've got a gel comparing the lack of gDNA as shown in the advertising pamphlet. What can I say, except: I love this product!“

- R.K. CSU

“Direct-zol is the most excellent kit for RNA isolation that I ever used in the past 20 years.”

- H.Z. (Harvard Medical School)

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Cat # Name Size Price
E1010-1-16 DNA Digestion Buffer 16 mL $31.90
R2050-1-200 TRI Reagent 200 ml $248.60
E1010-1-4 DNA Digestion Buffer 4 mL $16.50
C2003 Elution Plate 2 Plates $20.90
C2002 Collection Plate 2 Plates $24.20
C2007-2 96-Well Plate Cover Foil 2 Foils $10.00
C2007-4 96-Well Plate Cover Foil 4 Foils $10.00
C2004 Zymo-Spin I-96 Plate 2 Plates $156.20
W1001-30 DNase/RNase-Free Water 30 ml $24.20
W1001-10 DNase/RNase-Free Water 10 ml $20.90
R2050-2-160 Direct-zol RNA PreWash (Concentrate) 160 ml $182.60
R2054 Direct-zol-96 RNA 2 x 96 preps $459.80
R2056 Direct-zol-96 RNA 4 x 96 preps $742.50
R1003-3-48 RNA Wash Buffer 48 ml $115.50
E1010 DNase I Set 250 U $61.60