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Direct-zol-96 RNA Kits

R2054 / R2055 / R2056 / R2057


Direct-zol-96 RNA Kits

R2054 / R2055 / R2056 / R2057

Cat # Name Size Price Quantity
R2054 Direct-zol-96 RNA 2 x 96 preps $418.00
$313.50
+ -
R2055 Direct-zol-96 RNA (Product Supplied w/ 200 ml x 1 TRI Reagent) 2 x 96 preps $628.00
$471.00
+ -
R2056 Direct-zol-96 RNA 4 x 96 preps $675.00
$506.25
+ -
R2057 Direct-zol-96 RNA (Product Supplied w/ 200 ml x 2 TRI Reagent) 4 x 96 preps $1,105.00
$828.75
+ -

Documents


Isolate total RNA and small/miRNA from TRIzol without phase separation

Highlights


  • Easy Handling: Bypass chloroform, phase separation and precipitation steps.
  • NGS-Ready: Ultra-pure RNA without phenol carryover. No DNA contamination (DNase I included).
  • Non-Biased: Complete RNA recovery without miRNA loss.

Description


The Direct-zol-96 RNA Kits are RNA purification kits that provide a streamlined method for the purification of up to 10 µg (per well) of high-quality RNA directly from samples in TRI Reagent®. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.) using this product. The extraction method inactivates viruses and other infectious agents. The procedure is easy: simply apply a sample in TRI Reagent® to the Zymo-Spin I-96 Plate, then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including RT-PCR, transcription profiling, hybridization, etc. The entire procedure typically takes about 30 minutes (per 2 plates).

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Technical Specifications


Compatibility TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®.
Equipment Microplate centrifuge, vortex
Sample Inactivation TRI Reagent® (provided with R2055, R2057) inhibits RNase activity and inactivates viruses and other infectious agents.
Sample Source Any sample stored and preserved in TRI Reagent®, TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)).
Size Range Total RNA ≥ 17 nt
Yield 10 µg RNA (binding capacity), ≥10 µl (elution volume)

Product FAQ


Q1: Is DNase I available for individual purchase?

Q2: How to store DNase-I following resuspension?

Q3: Is the DNase-I treatment necessary?

Q4: Is the kit compatible with samples stored in DNA/RNA Shield?

Q5: Is Direct-zol suitable for very small numbers of cells?

Q6: Is it possible to extract proteins with the Direct-zol RNA kits?

Q7: Can samples be stored in TRIzol/TRI Reagent prior to processing?

Q8: Is it possible to isolate DNA with the Direct-zol RNA kits?

Q9: Is the RNA suitable for Next-Gen sequencing or other sensitive downstream applications?

Q10: Which phenol-based reagents are compatible with Direct-zol?

Q11: What is the difference between the Direct-zol RNA and Quick-RNA kits?

Q12: What is the difference between the Direct-zol RNA MiniPrep and the Direct-zol RNA MiniPrep Plus?

Q13: I ran out of RNA Wash Buffer. Can I use something else?

Product Video


Citations


Researchers used the Direct-zol RNA MiniPrep for heterologous E. coli expression of the FnCpf1 locus by means of RNA-seq and discovered a new endonuclease, Cpf1, which further improves upon the widely used CRISPR-Cas9 genome editing system. Cpf1 targets distinct PAM sequences, requires no tracrRNA, and cleaves DNA with staggered overhangs.

Zetsche B, et. al. (2015). Cpf1 is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell. 163(3): 759-71.

The Direct-zol RNA Miniprep kit was used to isolate pure total RNA from Rat cells, and the high quality RNA was used to create a cDNA library for RT-qPCR analysis of several genes related to hematopoietic Stem Cell (HSC) Development. The consistent RNA extraction allowed the scientists to determine that HSC cells do not significantly contribute to kidney repair following acute kidney injuries.

Burst V. et al. (2012) Survival and distribution of injected haematopoietic stem cells in acute kidney injury. Nephrol Dial Transplant 0: 1–8

High-quality RNA isolated with the Direct-zol™ RNA MiniPrep from fecal and culture samples were shown to be instrumental in the development of a rotavirus early detection system using RT-PCR. The efficient RNA isolation helps in identification of severe gastroenteritis in infants and young children.

Yoshiki F, Takashi S, Takagi H, et al. Amplification of all 11 RNA segments of group A rotaviruses based on reverse transcription polymerase chain reaction Microbiol Immunol 2012; 56:

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Reviews


“No phase separation was needed, but you still had the benefits of a Trizol extraction. No need to precipitate and resuspend samples, which means less sample loss during purification.”

- Adina B. (University of Guelph)

“This kit is amazing, I've got a gel comparing the lack of gDNA as shown in the advertising pamphlet. What can I say, except: I love this product!“

- R.K. CSU

“Direct-zol is the most excellent kit for RNA isolation that I ever used in the past 20 years.”

- H.Z. (Harvard Medical School)

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Kit Components