Quick-DNA Fecal/Soil Microbe 96 Kit

D6011


Quick-DNA Fecal/Soil Microbe 96 Kit

D6011

Cat # Name Size Price Quantity
D6011 Quick-DNA Fecal/Soil Microbe 96 Kit 2 x 96 Preps $673.00
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Documents


Datasheet: PDF   |   Protocol: PDF   |   SDS (MSDS): PDF   |  
Extract high quality, inhibitor-free metagenomic DNA from feces, soil, water, etc.

Highlights


  • Rapid, high-throughput (96-well) method for the isolation of inhibitor-free, PCR-quality DNA from fecal samples in minutes, including those from humans, birds, rats, mice, cattle, etc.
  • Ultra-high density BashingBeads are fracture resistant and chemically inert.
  • Zymo-Spin plate unique filtration technologies effectively removes PCR inhibitors from the DNA product.

Description


The Quick-DNA Fecal/Soil Microbe 96 Kit is designed for the simple and rapid isolation of inhibitor-free, PCR-quality host cell and microbial DNA from a variety of sample sources, including humans, birds, rats, mice, cattle, etc. The procedure is easy and can be completed in minutes: fecal samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads. Zymo-Spin technology is then used to isolate the DNA which is subsequently filtered to remove humic acids/polyphenols that can inhibit PCR. Eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, genotyping, methylation detection, etc.

Technical Specifications


Applicable For All sensitive downstream applications such as qPCR and Next-Generation Sequencing.
Elution Volume ≥ 50 µl
Equipment Centrifuge w/ microplate carriers, 96-well plate/block disruptor or pulverizer.
Processing Volume ≤80mg feces, ≤ 135 mg soil, ≤20 mg fungal/bacterial cells (wet weight)
Purity A260/A280 nm ≥1.8.
Sample Source Feces or soil
Sample Storage DNA stored at ≤ -20°C.
Size Range Capable of recovering genomic DNA up to and above 40 kb. Typical fragment sizes range from 25 kb - 35 kb. If present, parasitic and viral DNA will also be recovered.
Type Total DNA
Yield ≤ 5 µg total DNA

Product FAQ


Q1: My lysate seems viscous. What is causing this to happen? How can I fix this?

Q2: What is the purpose of Silicon-A-HRC Plate step?

Q3: Is it necessary to add beta-mercaptoethanol? Can this step be substituted or omitted?

Q4: When can an RNase A treatment be implemented in the protocol?

Kit Components