Quick-DNA/RNA Microprep Plus Kit

D7005 / D7005T


Quick-DNA/RNA Microprep Plus Kit

Cat # Name Size Price Quantity
D7005 Quick-DNA/RNA Microprep Plus Kit 50 Preps $438.90
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D7005T Quick-DNA/RNA Microprep Plus Kit 10 preps $119.90
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Quick-DNA/RNA Microprep Plus Kit

Highlights
  • Quick & Easy: Extract DNA and RNA from any sample including cells, solid tissue, whole blood, biological liquids, FFPE tissue, environmental (plant/seed), swabs (stool, soil, microbial samples), etc.
  • Sensitive: Single cell-level recovery of DNA and RNA.
  • Ultra-Pure: Ready for Next-generation sequencing, RT-qPCR, arrays, etc. DNase I included (Plus kit and MagBead only)
Description

The Quick-DNA/RNA Microprep Plus kit combines Quick-DNA/RNA technology with the addition of DNA/RNA Shield, a unique preservation and lysis technology, and Proteinase K to enable easy, reliable, and rapid isolation from a wide range of sample sources, including cells, solid tissue, and whole blood. The procedure uses Zymo-Spin column technology that results in high-quality gDNA and total RNA, ready for any downstream application including reverse transcription, microarray, sequencing.

Equipment Microcentrifuge, vortex, and heat block/bath (55°C)
Purity DNA and RNA are ready for Next-Gen Sequencing, RT-PCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.
Sample Source Any cells, solid tissue, whole blood, biological fluids, FFPE tissue, and samples stored in DNA/RNA Shield, etc.
Size Range Genomic DNA ≥40 kb and Total RNA ≥17 nt
Yield ≤ 5 µg DNA and ≤ 10 µg RNA

Yes, the catalog number for the DNase I set (DNase and DNA Digestion Buffer) that we offer is E1010.

The kit is optimized with Zymo Research's DNase, however DNase from other manufacturers can be used. Follow the respective protocol for on-column DNase treatment. If the DNase does not have a protocol, proceed with in-tube DNase treatment post-extraction, then purify using the RNA Clean & Concentrator-5.

Aliquot and store at – 20°C. Minimize multiple freeze thaw cycles. Lyophilized DNase I is stable at room temperature.

Yes, the Quick-DNA/RNA MicroPrep Plus (#D7005) is capable of isolating from single cell inputs (picogram amounts). A sensitive quantification method is needed (e.g. Qubit, qPCR, etc.)

If the downstream application requires DNA-free RNA, we recommend performing the DNase I treatment.

Yes, proteins can be precipitated. Please refer to the protocol appendix.

Yes. Use 80% ethanol as a substitute. DNA/RNA Wash Buffer is also sold separately.

Yes, both DNA and RNA is high quality (A260/A280 >1.8, A260/A230 >1.8) and suitable for any downstream application, including NGS, qPCR, etc.

Yes, this kit will isolate small/micro RNA’s ≥ 17 nucleotides.

Yes, freeze samples at -80°C after samples are lysed/homogenized in DNA/RNA Lysis Buffer. Bring the sample to room temperature prior to RNA purification.

Yes, bring samples homogenized and stored in DNA/RNA Shield to room temperature (20-30ºC). Add 1 volume of DNA/RNA Lysis Buffer (1:1) and mix well. Proceed with DNA Purification.

“This kit was invaluable. I was able to get at least 1 ug of RNA and several hundred nanograms of DNA from each sample - all of it high quality. Much better than my previous experience with a different dual DNA/RNA isolation kit.”

- Julie P. (CU Anschutz Medical Campus)

“I usually recommend this kit for those who want to extract both DNA and RNA from a single sample. We have even had great quality and yield for single insect samples.”

- Robert B. (Harvard University)

"The kit is very economical for simultaneous extraction of high quality DNA and RNA from very small amount of biological samples. More particularly, RNA is of good quality that suits best for applications like next-generation sequencing (RNA-Seq)."

- Muhammad A. (Northwestern University)

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Cat # Name Size Price
R1200-25 DNA/RNA Shield (2X Concentrate) 25 ml $68.00
W1001-10 DNase/RNase-Free Water 10 ml $20.90
C1004-50 Zymo-Spin IC Columns 50 Pack $58.30
R1200-1-5 PK Digestion Buffer 5 ml $17.60
C1001-50 Collection Tubes 50 Pack $16.50
D7010-2-50 DNA/RNA Prep Buffer 50 ml $69.30
D7001-1-50 DNA/RNA Lysis Buffer 50 ml $86.90
D7010-3-24 DNA/RNA Wash Buffer (Concentrate) 24 ml $66.00
E1010-1-4 DNA Digestion Buffer 4 mL $16.50
C1103-50 Zymo-Spin IC-XM Columns 50 Pack $69.30