Quick-DNA/RNA Miniprep Plus Kit

D7003 / D7003T


Quick-DNA/RNA Miniprep Plus Kit

D7003 / D7003T

Cat # Name Size Price Quantity
D7003 Quick-DNA/RNA Miniprep Plus Kit 50 Preps $399.00
+ -
D7003T Quick-DNA/RNA Miniprep Plus Kit 10 Preps $97.00
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Documents


Isolate DNA and RNA from any sample

Highlights


  • Quick & Easy: Extract DNA and RNA from any sample including cells, solid tissue, whole blood, biological liquids, FFPE tissue, environmental (plant/seed), swabs (stool, soil, microbial samples), etc.
  • Ultra-Pure: Ready for Next-generation sequencing, RT-qPCR, arrays, etc. DNase I included (Plus kit and MagBead only)

Description


The Quick-DNA/RNA MiniPrep Plus kit combines Quick-DNA/RNA technology with the addition of DNA/RNA Shield, a unique preservation and lysis technology, and Proteinase K to enable easy, reliable, and rapid isolation from any biological sample including cells, solid tissue, and whole blood. The procedure uses Zymo-Spin column technology that results in high-quality genomic DNA and total RNA that is ready for any downstream application including reverse transcription, microarray, sequencing, etc.

Technical Specifications


Equipment Microcentrifuge, vortex, and heat block/bath (55°C)
Purity DNA and RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.
Sample Source Any cells, solid tissue, whole blood, biological fluids, FFPE tissue, environmental (plant/seed), swabs (stool, soil, microbial samples), samples stored in DNA/RNA Shield™, etc.
Size Range Genomic DNA ≥40 kb and Total RNA ≥17 nt
Yield 100 µg DNA and RNA (binding capacity), ≥50 µl (elution volume)

Product FAQ


Q1: How to purify RNA from samples stored in RNAlater?

Q2: Is the kit compatible with samples stored in DNA/RNA Shield?

Q3: Can samples be stored in DNA/RNA Lysis Buffer prior to processing?

Q4: What is the difference between the Quick-DNA/RNA Miniprep (D7001) and Quick DNA/RNA Miniprep Plus (D7003)?

Q5: Is DNase I available for individual purchase?

Q6: Can I use other DNase I enzymes (or sets)? (e.g. Qiagen DNase I)?

Q7: How to store DNase-I following resuspension?

Q8: Is Quick-DNA/RNA suitable for very small numbers of cells?

Q9: Is the kit compatible with blood samples stored in PAXgene Blood Tubes?

Q10: Is the DNase-I treatment necessary?

Q11: Will the kit isolate small RNAs?

Q12: Is it possible to extract proteins with the Quick-DNA/RNA kit?

Q13: I ran out of DNA/RNA Wash Buffer. Can I use something else?

Q14: Is the DNA and RNA suitable for Next-Gen Sequencing or other sensitive downstream applications?

Citations


Reviews


“This kit was invaluable. I was able to get at least 1 ug of RNA and several hundred nanograms of DNA from each sample - all of it high quality. Much better than my previous experience with a different dual DNA/RNA isolation kit.”

- Julie P. (CU Anschutz Medical Campus)

“I usually recommend this kit for those who want to extract both DNA and RNA from a single sample. We have even had great quality and yield for single insect samples.”

- Robert B. (Harvard University)

"The kit is very economical for simultaneous extraction of high quality DNA and RNA from very small amount of biological samples. More particularly, RNA is of good quality that suits best for applications like next-generation sequencing (RNA-Seq)."

- Muhammad A. (Northwestern University)

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Kit Components