Quick-RNA Midiprep Kit

R1056


Quick-RNA Midiprep Kit

R1056

Cat # Name Size Price Quantity
R1056 Quick-RNA Midiprep Kit 25 preps $277.00
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Documents


Isolate total RNA and small/miRNA from cells and soft tissue

Highlights


  • Broad Range: Extract total RNA (including small/micro RNAs) from any sample.
  • DNA-Free: Genomic DNA removal column and DNase I included.
  • NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.

Description


The Quick-RNA Midiprep Kit is one of the most innovative RNA isolation kits available, designed for the easy, reliable, and rapid isolation of up to 1 mg total RNA from cultured cells or tissue samples. The procedure combines a unique, single-step RNA extraction/binding buffer with Clean-Spin™ column technology to yield high quality RNA in about 10 minutes. The Quick-RNA™ Midiprep Kit allows for the efficient recovery of total RNA from 103 to 108 cells or tissue. The method is easy: simply add the provided ZR RNA Buffer to extract total RNA from the cells of interest then purify the RNA using the provided Zymo-Spin™ V-E Columns. The result is highly-concentrated, purified RNA that is suitable for subsequent RNA-based methods including RT-PCR, hybridization, etc.

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Technical Specifications


Equipment Microcentrifuge, vortex, vacuum manifold
Purity RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8.
Sample Source Cells or tissue samples, yeast, plant or bacteria. Compatible with DNA/RNA Shield™ and RNAlater™.
Size Range Total RNA ≥ 17 nt
Yield Midiprep: 1 mg RNA (binding capacity), ≥ 200 µl (elution volume)

Product FAQ


Q1: What is the difference between the Quick-RNA Miniprep and the Quick-RNA Miniprep Plus?

Q2: Is DNase I available for individual purchase?

Q3: Is the DNase-I treatment necessary?

Q4: How to store DNase-I following resuspension?

Q5: I ran out of RNA Wash Buffer. Can I use something else?

Q6: Will the kit isolate small RNAs?

Q7: Can samples be stored in RNA Lysis Buffer prior to processing?

Q8: What is the difference between the Direct-zol RNA and Quick-RNA kits?

Q9: Is it possible to extract proteins with the Quick-RNA kit?

Reviews


"RNA isolation from tissue culture cells used to be my most hated protocol - labor intensive, tedious, and the horrible smell of the BME used in the protocol. This kit is much faster and easier and no horrible smells! Both the RNA yield and the real time results are just as good as our previous kit."

- Lisa G. (University of Virginia)

“Amazing results, the only RNA extraction kit I ever buy now. In my opinion, there is no product on the market that is as good a value and as effective as this kit for plant tissue RNA extractions.”

- Erin B. (Occidental College)

"Good price and easy to use, plus good quality. It is compatible to QIAGEN products RNeasy kit, but with reasonable price and equal quality. Especially the DNase is inexpensive and is included in the kit. No matter animal RNA, or plant RNA, or bacterial RNA, all could use with this one kit."

- Xiaolu J. (Florida Atlantic University)

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Kit Components