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ZymoBIOMICS RNA Miniprep Kit
Highlights
- Unbiased Lysis: Efficient and unbiased lysis of microbes including Grampositive/negative bacteria, fungi, protozoans, and viruses from any sample (feces, soil, plant, water, biofilms, swabs, saliva, body fluids, etc.)
- Ultra-Pure: Total RNA (including small/micro RNAs) is inhibitor-free and ready for qPCR and microbiome measurements using Next-Gen Sequencing.
- High Sensitivity: Increased detection limit of very low abundance organisms.
Original Manufacturer
Innovated in California, Made in the USA
Satisfaction 100% guaranteed, read Our Promise
ZymoBIOMICS RNA Miniprep Kit
Highlights
- Unbiased Lysis: Efficient and unbiased lysis of microbes including Grampositive/negative bacteria, fungi, protozoans, and viruses from any sample (feces, soil, plant, water, biofilms, swabs, saliva, body fluids, etc.)
- Ultra-Pure: Total RNA (including small/micro RNAs) is inhibitor-free and ready for qPCR and microbiome measurements using Next-Gen Sequencing.
- High Sensitivity: Increased detection limit of very low abundance organisms.
Original Manufacturer
Innovated in California, Made in the USA
Satisfaction 100% guaranteed, read Our Promise
| Cat # | Name | Size | Price | Quantity |
|---|---|---|---|---|
| R2001 | ZymoBIOMICS RNA Miniprep Kit | 50 Preps | $495.90 |
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| Cat # | Name | Size | Price | |
|---|---|---|---|---|
| D4300-7-30 | ZymoBIOMICS HRC Prep Solution | 30 ml | $114.30 | |
| W1001-30 | DNase/RNase-Free Water | 30 ml | $25.60 | |
| R1100-50 | DNA/RNA Shield | 50 ml | $72.10 | |
| R1003-3-24 | RNA Wash Buffer | 24 ml | $68.80 | |
| R1060-1-50 | RNA Lysis Buffer | 50 ml | $92.20 | |
| C1006-50-G | Zymo-Spin IIICG Columns | 50 Pack | $64.20 | |
| R1060-2-25 | RNA Prep Buffer | 25 ml | $46.70 | |
| C1001-50 | Collection Tubes | 50 Pack | $17.50 | |
| C1058-50 | Zymo-Spin III-HRC Filters | 50 Pack | $126.10 | |
| E1010-1-4 | DNA Digestion Buffer | 4 mL | $17.50 | |
| S6012-50 | ZR BashingBead Lysis Tubes (0.1 & 0.5 mm) | 50 Tubes | $117.80 | |
Description
Performance
Technical Specifications
| Equipment | Microcentrifuge, vortex, cell disrupter (recommended) |
|---|---|
| Purity | RNA is ready for Next-Gen sequencing, RTPCR, microarray, hybridization, etc. A260/A280, A260/A230: >1.8. |
| Sample Source | Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host RNA is efficiently isolated from ≤ 200 mg of mammalian feces, ≤ 250 mg soil, ≤ 200 mg plant/seed, 50-100 mg (wet weight) fungal bacterial cells, biofilms, and water. |
| Size Range | Total RNA ≥17 nt |
| Yield | 100 µg RNA (binding capacity), ≥50 µl (elution volume) |
Resources
FAQ
Environmental samples often contain inhibitors such as polyphenolics, humic/fulvic acids, tannins, melanins, etc. that affect downstream applications such as PCR. The Zymo-Spin III-HRC is designed to remove these PCR inhibitors and does not bind DNA/RNA. The Zymo-Spin III-HRC can be purchased separately as the OneStep PCR Inhibitor Kit (D6030).
Sample overloading can cause nucleic acids to precipitate and crash out of solution, reducing yields. Use less sample input to avoid precipitation.
This is a normal occurrence as DNA/RNA Shield contains detergents. To reduce foam, centrifuge at 12,000 x g in 1 minute intervals before transferring the supernatant.
This can happen on occasion due to transport or storage at lower temperatures. The reagent functionality is not affected; however, the precipitate can be resolved by heating the reagent to >37 °C.
Citations
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