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Recover highly concentrated pure DNA from Agarose Gels:
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Zyppy Plasmid Miniprep Kit
Purify ultra-pure plasmid DNA from 600 µl of overnight E. coli culture without pelleting cells.
Highlights
- Fastest: 8 minutes from culture flask to high-quality plasmid DNA.
- Pellet-Free: Direct lysis procedure omits cell-pelleting and resuspension steps.
- High Quality: Ready for PCR, sequencing, cloning, and transfection.
Original Manufacturer
Innovated in California, Made in the USA
Satisfaction 100% guaranteed, read Our Promise
Highlights
- Fastest: 8 minutes from culture flask to high-quality plasmid DNA.
- Pellet-Free: Direct lysis procedure omits cell-pelleting and resuspension steps.
- High Quality: Ready for PCR, sequencing, cloning, and transfection.
Original Manufacturer
Innovated in California, Made in the USA
Satisfaction 100% guaranteed, read Our Promise
| Cat # | Name | Size | Price | Quantity |
|---|---|---|---|---|
| D4019 | Zyppy Plasmid Miniprep Kit | 100 Preps | $134.80 |
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| D4020 | Zyppy Plasmid Miniprep Kit | 400 Preps | $456.60 |
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| D4036 | Zyppy Plasmid Miniprep Kit | 50 Preps | $80.40 |
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| D4037 | Zyppy Plasmid Miniprep Kit | 800 Preps | $828.20 |
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| Cat # | Name | Size | Price | |
|---|---|---|---|---|
| C1019-50 | Zymo-Spin IIN Columns | 50 Pack | $63.00 | |
| C1001-50 | Collection Tubes | 50 Pack | $17.50 | |
| D4036-3-30 | Endo-Wash Buffer | 30 ml | $21.00 | |
| D4036-2-160 | Neutralization Buffer (Yellow) | 160 ml | $148.20 | |
| D4036-2-20 | Neutralization Buffer (Yellow) | 20 ml | $14.00 | |
| D4036-3-15 | Endo-Wash Buffer | 15 ml | $11.60 | |
| D4036-1-12 | 7X Lysis Buffer (Blue) | 12 ml | $40.90 | |
| D4036-1-48 | 7X Lysis Buffer (Blue) | 48 ml | $80.50 | |
| D4036-1-6 | 7X Lysis Buffer (Blue) | 6 ml | $17.50 | |
| D4036-2-40 | Neutralization Buffer (Yellow) | 40 ml | $40.90 | |
| D4036-3-120 | Endo-Wash Buffer | 120 ml | $57.20 | |
| D4036-4-12 | Zyppy Wash Buffer (Concentrate) | 12 ml | $21.00 | |
| D4036-4-48 | Zyppy Wash Buffer (Concentrate) | 48 ml | $57.20 | |
| D4036-4-6 | Zyppy Wash Buffer (Concentrate) | 6 ml | $11.60 | |
| D4036-5-20 | Zyppy Elution Buffer | 20 ml | $29.10 | |
| D4036-5-5 | Zyppy Elution Buffer | 5 ml | $10.50 | |
Description
Performance
Technical Specifications
| Applicable For | Ligation, sequencing, restriction endonuclease digestion, in vitro transcription, and other sensitive applications requiring pure DNA. |
|---|---|
| Elution Volume | ≥ 30 µl |
| Equipment | Microcentrifuge and/or a vacuum manifold. |
| Processing Time | 8 min |
| Purity | Typical Abs260/280 ≥1.8. |
| Size Range | Up to 25 kb |
| Yield | Up to 25 µg of plasmid DNA per preparation, depending on the plasmid copy number, culture growth conditions, and strain of E. Coli utilized. |
Resources
FAQ
Zyppy Wash Buffer is available for purchase, separately. However, you can substitute with DNA Wash Buffer, Plasmid Wash Buffer, or 80% ethanol.
≤ 50 Endotoxin Units/µg of plasmid DNA.
No, we recommend using our ZR BAC DNA Miniprep Kit or ZymoPURE II Kits for isolating large constructs.
Yes, all our plasmid preps have an endo-wash step to remove endotoxins and produce plasmid DNA that is ready for transfection with most cell lines.
10 mM Tris-HCl, 0.1 mM EDTA, pH 8.5.
Yes, pellet and resuspend E.coli in water or TE before proceeding with the protocol.
Reviews
"The speed I was doing a side by side comparison with Invitrogen and it is really nice to go from culture to plasmid DNA in less than 15 min. Also the precipitate was really solid, no floaties, so it was very easy to pipet off the supernatant."
Rebecca N.
"It performs just as well as the Qiagen mini-prep kit (the accepted lab-standard). The kit was very easy to follow and the resultant DNA was compatible with our complex downstream procedures."
Richard F.
"The results gave what they promised! Clean DNA."
Pamela R.
UT Medicine San Antonio
Citations
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