- Easy Handling: Bypass chloroform, phase separation and precipitation steps.
- NGS-Ready: Ultra-pure RNA without phenol carryover. No DNA contamination (DNase I included).
- Non-Biased: Complete RNA recovery without miRNA loss.
|Compatibility||TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®.|
|Sample Inactivation||TRI Reagent® (provided with R2051, R2053) inhibits RNase activity and inactivates viruses and other infectious agents.|
|Sample Source||Any sample stored and preserved in TRI Reagent®, TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)).|
|Size Range||Total RNA ≥ 17 nt|
|Yield||50 µg RNA (binding capacity), ≥25 µl (elution volume)|
Q1: Is DNase I available for individual purchase?
Q2: How to store DNase-I following resuspension?
Q3: Is the kit compatible with samples stored in DNA/RNA Shield?
Q4: Is Direct-zol suitable for very small numbers of cells?
Q5: Is it possible to extract proteins with the Direct-zol RNA kits?
Q6: Is the DNase-I treatment necessary?
Q7: Can samples be stored in TRIzol/TRI Reagent prior to processing?
Q8: Is it possible to isolate DNA with the Direct-zol RNA kits?
Q9: Is the RNA suitable for Next-Gen sequencing or other sensitive downstream applications?
Q10: Which phenol-based reagents are compatible with Direct-zol?
Q11: What is the difference between the Direct-zol RNA and Quick-RNA kits?
Q12: What is the difference between the Direct-zol RNA MiniPrep and the Direct-zol RNA MiniPrep Plus?
Q13: I ran out of RNA Wash Buffer. Can I use something else?
Researchers used the Direct-zol RNA MiniPrep for heterologous E. coli expression of the FnCpf1 locus by means of RNA-seq and discovered a new endonuclease, Cpf1, which further improves upon the widely used CRISPR-Cas9 genome editing system. Cpf1 targets distinct PAM sequences, requires no tracrRNA, and cleaves DNA with staggered overhangs.Zetsche B, et. al. (2015). Cpf1 is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell. 163(3): 759-71.
The Direct-zol RNA Miniprep kit was used to isolate pure total RNA from Rat cells, and the high quality RNA was used to create a cDNA library for RT-qPCR analysis of several genes related to hematopoietic Stem Cell (HSC) Development. The consistent RNA extraction allowed the scientists to determine that HSC cells do not significantly contribute to kidney repair following acute kidney injuries.Burst V. et al. (2012) Survival and distribution of injected haematopoietic stem cells in acute kidney injury. Nephrol Dial Transplant 0: 1–8
High-quality RNA isolated with the Direct-zol™ RNA MiniPrep from fecal and culture samples were shown to be instrumental in the development of a rotavirus early detection system using RT-PCR. The efficient RNA isolation helps in identification of severe gastroenteritis in infants and young children.Yoshiki F, Takashi S, Takagi H, et al. Amplification of all 11 RNA segments of group A rotaviruses based on reverse transcription polymerase chain reaction Microbiol Immunol 2012; 56:
“No phase separation was needed, but you still had the benefits of a Trizol extraction. No need to precipitate and resuspend samples, which means less sample loss during purification.”
- Adina B. (University of Guelph)
“This kit is amazing, I've got a gel comparing the lack of gDNA as shown in the advertising pamphlet. What can I say, except: I love this product!“
- R.K. CSU
“Direct-zol is the most excellent kit for RNA isolation that I ever used in the past 20 years.”
- H.Z. (Harvard Medical School)