Quick COVID-19 Antibody Detection Kit


Quick COVID-19 Antibody Detection Kit

Cat # Name Size Price Quantity
D5327 Quick COVID-19 Antibody Detection Kit 96 rxns $483.00
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Quick COVID-19 Antibody Detection Kit Product Photo

Highlights

  • Highly specific detection of IgG antibodies directed SARS-CoV-2 receptor binding domain (RBD)
  • Set up to results in only 2.5 hours
  • Included Cut-off Control simplifies test validation
Description

The Quick COVID-19 Antibody Detection Kit provides a straightforward ELISA test for COVID-19 Antibody Detection in human serum or plasma. The Quick COVID-19 Antibody Detection Kit provides results within 2.5 hours after sample preparation and is directly validated by the included controls. The Cut-off Control represents a reliable threshold value between negative and positive samples, thus allowing for rapid test evaluation.


Detection Absorbance at 450 nm with a reference filter between 560 nm and 650 nm.
Processing Time 2.5 hours
Required Equipment Incubator capable of reaching 37°C; Plate reader; Multi-channel pipette is recommended.
Sample Quality A minimum of 2 µl human serum/plasma per well.
Sample Sources Human serum or plasma containing anticoagulants such as Citrate, EDTA or Heparin (heat-inactivated or untreated).

Q1: Why is there no color development after addition of the TMB Substrate and Stop Solution?

Make sure that correct buffers and reagents were used to wash the plate and dilute the samples. 2. Make sure that the 1X ELISA Wash Buffer was prepared according to the instructions listed in the protocol. 3. Make sure that all components were stored at 4 °C.

Q2: What is the shelf life of the kit?

We guarantee the integrity of kit components for up to 6 months from date of purchase.

Q3: How to store the components of the Quick COVID-19 Antibody Detection Kit?

The components are shipped on blue ice. Upon arrival, immediately put all the kit components to 4 °C.

Q4: Where are the controls derived from?

Negative Control contains human negative serum (heat-inactivated) diluted 1:50 in Sample Diluent. Positive Control contains human negative serum (heat-inactivated) diluted 1:50 in Sample Diluent spiked with an antibody directed against the coated antigen. Cut-off Control contains an antibody directed against the coated antigen diluted in Sample Diluent.

Q5: The controls do not give the expected ODs listed in the protocols. What could be the reason for that?

Allow all reagents to warm up to room temperature for 30 minutes prior to starting. 2. Make sure that all controls have been mixed well before adding them to the plate. 3. Stick to the order of the protocol and add the controls after the samples right before incubating the plate at 37 °C for 1 h. 4. After adding the TMB Substrate, make sure that the Stop Solution is added after 10 minutes. Determine OD immediately after adding the Stop Solution. 5. Double-check the absorbance filters used in the plate reader. Use 450 nm for measuring with a reference filter between 560 nm and 650 nm.


Cat # Name Size Price
D5327-8-11 Stop Solution 11 ml $14.00
D5327-6-11 Anti-IgG HRP Antibody 11 ml $110.00
D5327-5-500 Cut-off Control 500 µl $132.00
D5327-4-500 Positive Control 500 µl $120.00
D5327-3-500 Negative Control 500 µl $102.00
D5327-2-11 Sample Diluent 11 ml $14.00
D5327-1-15 10X ELISA Wash Buffer 15 ml $20.00