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    RNA Clean & Concentrator-100

    R1019


    RNA Clean & Concentrator-100

    Cat # Name Size Price Quantity
    R1019 RNA Clean & Concentrator-100 25 Preps $214.10
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    Highlights

    • NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.
    • Ultra-Pure: Eliminate contaminants and inhibitors in 5 minutes.
    • Maximum Recovery: Recover > 90% and elute in as little as 100 µl.
    Description

    The RNA Clean & Concentrator-100 kit are RNA clean up kits that provide a simple and reliable method for the rapid preparation of high-quality, RT-PCR-ready RNA. This simple procedure is based on the use of a unique single-buffer system and Zymo-Spin column technology that allows for selective recovery of total RNA (> 17 nt), large RNAs (> 200 nt), and/or small RNAs (17-200 nt). The procedure is easy: Add binding buffer and ethanol to your sample, then bind, wash and elute ultra pure RNA. The RNA can be eluted from the Zymo-Spin V-E Column in as little as ≥ 100 µl of RNase-free water. The highly-concentrated, purified RNA is suitable for all subsequent analyses and molecular manipulations.

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    Equipment Microcentrifuge and Vacuum Manifold
    Format Spin-Column
    Purity RNA is ready for Next-Gen sequencing, RT-PCR, microarray, hybridization, etc. A260/A280, A260/A230: > 1.8.
    Sample Source DNase I treated RNA, in vitro transcription products, the aqueous phase following TRIzol/chloroform or similar extraction
    Size Range Total RNA ≥ 17 nt
    Supplemental Info
    Yield 1 mg RNA (binding capacity), ≥ 100 µl (elution volume)

    Yes. The kit efficiently recovers all types of nucleic acids.

    Carrier RNA can be added with no detrimental effects. The RCC is highly efficient without the need for carrier RNA, and no significant improvement in recovery has been observed with carrier RNA.

    Yes, the kit efficiently removes unincorporated fluorescent dyes, radiolabeled dNTP’s and Biotin.

    Product Tolerance Reference: – ≤5% Triton X-100 – ≤5% Tween-20 – ≤5% Sarkosyl – ≤0.1% SDS – ≤90% formamide – Sucrose samples should be diluted/titrated down 10- to 100- fold.

    Yes, follow respective protocol for on-column DNase treatment. If the DNase does not have a protocol, proceed with in-tube DNase treatment post clean-up, then re-purify.

    If the downstream application requires DNA-free RNA, we would recommend performing in-column or in-tube DNase I treatment.

    Both treatments will remove DNA efficiently. For pre-extracted RNA, perform in-tube DNase treatment followed by RNA clean-up.



    "Great for purifying small amounts of RNA. I use a bioanalyzer after and I can see the difference from before, cleaner bands and the concentration increases with a smaller amount of elutant."

    - Catherine A. (USAMRIID)

    “Kit was easy and fast, and cleaned up my RNA samples very well. It did exactly what I needed it to do.”

    - Caroline S. (Cornell University)

    "The product is very easy to use, very quick protocol for an excellent RNA clean up. It also purifies small RNA which is not the case of all RNA clean up columns."

    - Agnes N. (Biofidal)

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    Cat # Name Size Price
    W1001-10 DNase/RNase-Free Water 10 ml $21.50
    R1060-2-10 RNA Prep Buffer 10 ml $20.40
    C1001-50 Collection Tubes 50 Pack $17.00
    R1003-3-6 RNA Wash Buffer 6 ml $20.40
    R1013-2-100 RNA Binding Buffer 100 ml $145.00