RNA Clean & Concentrator-5

R1013 / R1014 / R1015 / R1016


RNA Clean & Concentrator-5

R1013 / R1014 / R1015 / R1016

Cat # Name Size Price Quantity
R1013 RNA Clean & Concentrator-5 (DNase Included) 50 Preps $170.00
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R1014 RNA Clean & Concentrator-5 (DNase Included) 200 Preps $585.00
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R1015 RNA Clean & Concentrator-5 (DNase not Included) 50 Preps $142.00
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R1016 RNA Clean & Concentrator-5 (DNase not Included) 200 Preps $485.00
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Documents


RNA clean-up from any reaction

Highlights

  • NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.
  • Ultra-Pure: Eliminate contaminants and inhibitors in 5 minutes.
  • Maximum Recovery: Recover >90% and elute in as little as 6 μl.
Description

The RNA Clean & Concentrator kits are RNA clean up kits that provide a simple and reliable method for the rapid preparation of high-quality RT-PCR-ready, DNA-free (R1013, R1014) RNA. This simple procedure is based on the use of a unique single-buffer system and Zymo-Spin column technology that allows for selective recovery of total RNA (> 17 nt), large RNAs (> 200 nt), and/or small RNAs (17-200 nt). The procedure is easy: Add binding buffer and ethanol to your sample, then bind, wash and elute ultra pure RNA. The RNA can be eluted from the Zymo-Spin IC Column in as little as ≥ 6 µl of RNase-free water. The highly-concentrated, purified RNA is suitable for all subsequent analyses and molecular manipulations.

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Equipment Microcentrifuge
Format Spin-Column
Purity RNA is ready for Next-Gen sequencing, RT-PCR, microarray, hybridization, etc. A260/A280, A260/A230: > 1.8.
Sample Source DNase I treated RNA, in vitro transcription products, the aqueous phase following TRIzol/chloroform or similar extraction
Size Range Total RNA ≥ 17 nt
Yield 10 µg RNA (binding capacity), ≥ 6 µl (elution volume)

Q1: Can the RCC kit be used to purify DNA (cDNA)?

Yes. The kit efficiently recovers all types of nucleic acids.

Q2: Should carrier RNA be added to the sample prior to purification?

Carrier RNA can be added with no detrimental effects. The RCC is highly efficient without the need for carrier RNA, and no significant improvement in recovery has been observed with carrier RNA.

Q3: Will this kit remove fluorescent dyes, radiolabeled dNTP’s and/or Biotin?

Yes, the kit efficiently removes unincorporated fluorescent dyes, radiolabeled dNTP’s and Biotin.

Q4: Are samples containing high concentration of detergents, salts, formamide or sucrose compatible with the RCC buffer system?

Product Tolerance Reference: – ≤5% Triton X-100 – ≤5% Tween-20 – ≤5% Sarkosyl – ≤0.1% SDS – ≤90% formamide – Sucrose samples should be diluted/titrated down 10- to 100- fold.

Q5: Can other DNase I enzymes or sets (e.g. Qiagen DNase I) be used?

Yes, follow respective protocol for on-column DNase treatment. If the DNase does not have a protocol, proceed with in-tube DNase treatment post clean-up, then re-purify.

Q6: Is DNase I treatment necessary?

If the downstream application requires DNA-free RNA, we would recommend performing in-column or in-tube DNase I treatment.




“It isolates small RNAs in the same go, unlike MinElute columns which leaves out RNAs smaller than 200nt.”

- Anupma C.

“The Zymo-Spin IC column is convenient.”

- Lihua D. (National Institutes of Health)

“Kit was easy and fast, and cleaned up my RNA samples very well. It did exactly what I needed it to do.”

- Caroline S. (Cornell University)

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Cat # Name Size Price
W1001-10 DNase/RNase-Free Water 10 ml $19.00
W1001-4 DNase/RNase-Free Water 4 ml $12.00
R1003-3-12 RNA Wash Buffer 12 ml $30.00
R1003-3-24 RNA Wash Buffer 24 ml $59.00
R1013-2-100 RNA Binding Buffer 100 ml $128.00
R1013-2-25 RNA Binding Buffer 25 ml $49.00
R1060-2-100 RNA Prep Buffer 100 ml $122.00
R1060-2-25 RNA Prep Buffer 25 ml $40.00
C1001-50 Collection Tubes 50 Pack $15.00
C1004-50 Zymo-Spin IC Columns 50 Pack $53.00
E1010-1-4 DNA Digestion Buffer 4 mL $15.00