RNA Clean & Concentrator-25
R1017 / R1018
- NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.
- Ultra-Pure: Eliminate contaminants and inhibitors in 5 minutes.
- Maximum Recovery: Recover >90% and elute in as little as 25 μl.
|Purity||RNA is ready for Next-Gen sequencing, RT-PCR, microarray, hybridization, etc. A260/A280, A260/A230: > 1.8.|
|Sample Source||DNase I treated RNA, in vitro transcription products, the aqueous phase following TRIzol/chloroform or similar extraction|
|Size Range||Total RNA ≥ 17 nt|
|Yield||50 µg RNA (binding capacity), ≥ 25 µl (elution volume)|
Q1: Can the RCC kit be used to purify DNA (cDNA)?
Q2: Should carrier RNA be added to the sample prior to purification?
Q3: Will this kit remove fluorescent dyes, radiolabeled dNTP’s and/or Biotin?
Q4: Are samples containing high concentration of detergents, salts, formamide or sucrose compatible with the RCC buffer system?
Q5: Can other DNase I enzymes or sets (e.g. Qiagen DNase I) be used?
Q6: Is DNase I treatment necessary?
In experiments designed to investigate RNase E-mediated degradation of mRNAs in cyanobacteria, the RNA Clean & Concentrator-25 was used to both concentrate gel-purified in vitro transcribed RNA as well as to clean up total cellular RNA for reverse transcription following DNase treatment. The researchers found that regulatory antisense RNAs are able to protect mRNA from being degraded by RNase E through forming RNA-RNA complexes with their target mRNA molecules.Stazic et al. (2011) Antisense RNA protects mRNA from RNase E degradation by RNA–RNA duplex formation during phage infection. Nucleic Acids Res. 39(11), 4890–4899.
"Great for purifying small amounts of RNA. I use a bioanalyzer after and I can see the difference from before, cleaner bands and the concentration increases with a smaller amount of elutant."
- Catherine A. (USAMRIID)
“Kit was easy and fast, and cleaned up my RNA samples very well. It did exactly what I needed it to do.”
- Caroline S. (Cornell University)
"The product is very easy to use, very quick protocol for an excellent RNA clean up. It also purifies small RNA which is not the case of all RNA clean up columns."
- Agnes N. (Biofidal)
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