ZR-96 DNA Clean-Up Kit
D4017 / D4018
- Quick (20 minute), large-scale recovery of ultra-pure DNA from PCR, endonuclease digestions, cell-free lysates, etc.
- ZR-96 Silicon-A Plate design allows DNA to be eluted at high concentrations into minimal volumes of solvent.
- Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, etc.
The ZR-96 DNA Clean-up Kit is a PCR purification kit that provides for rapid, large-scale (96-well) purification and concentration of high-quality DNA from PCR samples, endonuclease digestions, or crude plasmid preparations. Simply add the specially formulated DNA Binding Buffer to your samples and transfer to the wells of the supplied Silicon-A Plate. There is no need for organic denaturants or chloroform. Instead, the product features Fast-Spin plate technology to yield high-quality, purified DNA in just minutes.
|Detergent Tolerance||≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS|
|Elution Volume||≥ 30 µl for shallow well, ≥ 10 µl for deep well|
|Equipment||Centrifuge with microplate carriers|
|Purity||A260/A280 > 1.8|
|Sample Source||DNA from PCR, endonuclease digestions, DNA modification reactions, isotope/fluorescence labeling reactions, etc.|
|Size Range||75 bp to 23 kb for shallow well, 50 bp to 23 kb for deep well|
|Yield||≤ 5 µg total DNA can be recovered. For DNA 75 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70%.|
Q1: What is the lower limit and minimal amount of DNA that can be recovered?
Q2: How to process naked DNA stored in DNA/RNA Shield?
Q3: What to do if ethanol addition to the DNA Wash Buffer was omitted?
Q4: What happens if more DNA was loaded on the columns than the stated maximum binding capacity?
Q5: How many times can columns be reloaded?
Q6: What is the minimum input volume of DNA sample?
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